| 2008年甘肃省部分地区虫媒病毒调查 |
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| 引用本文： | 李铭华,付士红,冯云,高晓艳,翟友刚,于德山,李国太,贾玉新,梁国栋. 2008年甘肃省部分地区虫媒病毒调查[J]. 疾病监测, 2010, 25(2): 103-106. DOI: 10.3784/j.issn.1003-9961.2010.02.007 |
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| 作者姓名： | 李铭华 付士红 冯云 高晓艳 翟友刚 于德山 李国太 贾玉新 梁国栋 |
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| 作者单位： | 1.中国疾病预防控制中心病毒病预防控制所 传染病预防控制国家重点实验室, 北京 100052; |
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| 基金项目： | 国家科技部重大传染病专项(No.2003BA712A08-01);;中美新发和再发传染病合作项目(No.U10-GH000004)~~ |
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| 摘 要： | 目的调查甘肃省部分地区虫媒病毒分布状况,为虫媒病毒病防治提供依据。方法2008年7-8月在甘肃省嘉峪关、张掖、酒泉、白银和平凉市采集蚊虫标本,用细胞培养法分离病毒,用血清学和分子生物学方法对病毒分离物进行鉴定;利用生物信息学技术对新分离病毒的序列进行同源性和系统发生分析。结果采集到3属280批共13839只蚊虫标本,从中分离到6株病毒,血清学和分子生物学鉴定结果显示6株病毒(GSBY0801、GSBY0804、GSBY0810、GSBY0816、GSBY0827、GSBY0861)均为基因Ⅰ型乙脑病毒。新分离株与减毒活疫苗株SA14-14-2相比,E基因核苷酸同源性为87.5%～87.9%,氨基酸同源性为96.8%～97.2%。结论甘肃省东部地区存在基因Ⅰ型乙脑病毒的流行,流行病毒株与中国四川省乙脑病毒分离株进化关系较近。
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| 关 键 词： | 虫媒病毒 乙型脑炎病毒 序列分析 种系发生 |
| 收稿时间： | 2009-11-02 |
Distribution of arbovirus in some areas of Gansu province,2008 |
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| LI Ming-hua,FU Shi-hong,FENG Yun,GAO Xiao-yan,ZHAI You-gang,YU De-shan,LI Guo-tai,JIA Yu-xin,LIANG Guo-dong.National Priority Laboratory for Infectious Disease Prevention , Control,Institute for Viral Disease Control , Prevention,Chinese Center for Disease Control , Prevention,Beijing ,China. Distribution of arbovirus in some areas of Gansu province,2008[J]. Disease Surveillance, 2010, 25(2): 103-106. DOI: 10.3784/j.issn.1003-9961.2010.02.007 |
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| Authors: | LI Ming-hua FU Shi-hong FENG Yun GAO Xiao-yan ZHAI You-gang YU De-shan LI Guo-tai JIA Yu-xin LIANG Guo-dong.National Priority Laboratory for Infectious Disease Prevention Control Institute for Viral Disease Control Prevention Chinese Center for Disease Control Prevention Beijing China |
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| Affiliation: | 1.National Priority Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China |
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| Abstract: | Objective To investigate the distribution of arbovirus in some areas of Gansu province,and provide evidence for the prevention and control of arbovirus disease.Methods Mosquitoes were collected in Jiayuguan,Zhangye,Jiuquan,Baiyin and Pingliang in 2008 and the viruses were isolated from them by cell culture.The isolates were identified by serological and molecular biological methods and the sequences were analyzed by bioinformatics.ResultsA total of 13 839 mosquitoes were collected.Six virus strains were iso... |
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| Keywords: | arbovirus Japanese encephalitis virus sequence analysis phylogeny |
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