应用流式细胞仪研究深圳地区健康成人及HIV/AIDS患者的免疫状况

目的：研究深圳地区健康人、HIV无症状感染者及AIDS患者的免疫状态，以了解艾滋病早期T细胞亚群的变化过程。方法：应用流式细胞仪三色荧光单克隆抗体（CD4－FITC／CD8－PE／CD3－PC5）检测34例正常健康成人，12例HIV感染无症状者，23例AIDS患者的外周血CD4^ 、CD8 ^ T淋巴细胞计数及CD4^ /CD8^ 比值。结果：HIV感染无症状者及AIDS患者的CD4^ 淋巴细胞及CD4^ /CD8比值均明显低于健康成人（P＜0．01）；HIV感染无症状者CD8^ T淋巴细胞明显高于正常健康人和AIDS患者（P＜0．01）；CD4^ T淋巴细胞随病程进展不断下降，AIDS患者与HIV感染无症状者之间存在着显著差异。结论：T淋巴细胞免疫状况可作为评价AIDS病程进展程度的重要指标。

Study of Immunological Status of HIV/AIDS Patients and Normal Adults in Shenzhen by Flow Cytometer

Objective:To study the immunological status of HIV/AIDS patients and normal adults in Shenzhen and the changing process of T cell subsets in early clinic stage of AIDS. Methods:T cell subsets from 34 normal adults, 12 asymptomatic HIV carriers, 20 AIDS patients were detected by flow cytometer. Results:CD + 4 cell conuts and CD + 4/CD + 8 ratios of HIV/AIDS patients were much less than normal adults ( P<0.01 ), thile an evident increasing of CD + 8 cell count has been found in asymptomatic HIV carriers. The remarkable difference( P<0.01 ) of CD + 4 cell count between AIDS patients and asymptomatic HIV carriers was indicated in the investigation. Conclusion:The immunolgical status of T cell might be considered as an important indicator in AIDS stage.