差示分光光度法测定安乃近片的含量

目的:建立测定安乃近片含量的新方法。方法:采用差示分光光度法。以0.1mol.L-1盐酸溶液为介质制备参比液,以0.1mol.L-1氢氧化钠溶液为介质制备样品液,通过在波长283nm处,测定2种溶液的差示吸收值直接测定安乃近的含量,并将结果与其他方法(紫外分光光度法、高效液相色谱法和碘量法)比较。结果:安乃近检测浓度线性范围为10.35～51.75μg.L-(1r=0.9998),平均回收率为99.94%,RSD=0.27%(n=5);4种方法含量测定结果基本一致。结论:建立的新方法测定结果准确,方法简便、专属性强,可用于该制剂的含量测定。

Content Determination of Metamizole Sodium Tablets by Differential Spectrophotometry

OBJECTIVE:To establish new method for the content determination of Metamizole sodium tablets.METHODS:The differential spectrophotometry was adopted.0.1 mol·L-1 hydrochloric acid and 0.1 mol·L-1 sodium hydroxide were used respectively as medium to prepare reference solution and sample solution.The content of metamizole sodium was determined by measuring differential absorption value of 2 kinds of solution at wavelength of 283 nm.Results of the study were compared with those of other methods(UV spectrophotometry,HPLC and iodometric method).RESULTS:The calibration curves showed good linearity in the range of 10.35～51.75 μg·L-1(r=0.999 8) with an average recovery of 99.94%(RSD=0.27%,n=5).Results of content determination of 4 methods were similar.CONCLUSION:Established method is accurate,simple,specific and suitable for the content determination of Metamizole sodium tablets.