红花檵木叶黄酮类化学成分的分离与鉴定

目的:研究红花檵木叶中的黄酮类化学成分。方法:采用D101大孔树脂分离富集红花檵木叶乙醇提取物中的黄酮类成分,应用硅胶柱、Sephadex LH-20凝胶柱和RP-ODS柱色谱分离化合物,并通过核磁共振光谱、紫外光谱和电喷雾质谱等谱学手段鉴定化合物结构。结果:从红花檵木叶乙醇提取物中分离鉴定了5个黄酮类化合物,分别为山柰酚、槲皮素、杨梅素、杨梅素-3-O-α-L-鼠李糖苷、异槲皮苷。所有黄酮类化合物均为首次从该植物中分离得到。结论:本试验结果可为红花檵木的进一步开发利用提供科学依据。

Isolation and Identification of Flavonoid Constituents from Loropetalum chinense

OBJECTIVE: To study the flavonoid constituents of Loropetalum chinense. METHODS: The flavonoid constituents were isolated and purified from ethanol extract of L. chinense by the column chromatography of D101 macroporous resin coupled with silica gel, Sephadex LH-20 and RP-ODS column chromatography. Their chemical structures of compound were elucidated by NMR, UV and ESI-MS spectral analysis. RESULTS: Five flavonoid constituents were isolated and identified as kaempferol (1), quercetin(2),myricetin(3),myricetin-3-O-α-L-rhamn-oside (4), isoquercetin (5). All of the flavonoid compounds were isolated from the plant for the first time. CONCLUSION: The results provide the scientific basis for further exploitation of L. chinense.