| 燃煤型氟中毒大鼠智力与脑ERK1/2表达关系 |
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| 引用本文： | 冉龙艳,桂传枝,吴昌学,何江,官志忠. 燃煤型氟中毒大鼠智力与脑ERK1/2表达关系[J]. 中国公共卫生, 2010, 26(10) |
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| 作者姓名： | 冉龙艳 桂传枝 吴昌学 何江 官志忠 |
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| 作者单位： | 1. 贵阳医学院病理学及分子生物学教研室,贵州贵阳,550004
2. 贵阳医学院公共卫生学院 |
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| 基金项目： | 科技部国际合作项目，国家自然科学基金，贵州省省长资金项目，高层次人才特助项目 |
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| 摘 要： | 目的探讨燃煤型氟中毒大鼠智力变化及其发生机制。方法复制燃煤型氟中毒大鼠模型,用Morris水迷宫检测大鼠行为学改变;蛋白印迹及实时荧光定量PCR检测信号转导系统细胞外调节蛋白激酶1/2(ERK1/2)表达水平。结果高、低氟组大鼠逃避潜伏时间分别为(14.44±10.18)和(11.64±7.08)s,较对照组明显延长(P0.05);氟中毒大鼠第7 d穿台次数及逗留平台相限时间均较对照组减少(P0.05);染氟组大鼠脑组织中ERK1/2磷酸化蛋白较对照组升高1.5~2.3倍(P0.05),mRNA表达水平较对照组升高2.0~3.5倍(P0.05),呈剂量-效应关系。结论燃煤型氟中毒可引起大鼠学习、记忆能力降低,其机制可能与脑组织中ERK/MAPK信号转导通路的激活有关。
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| 关 键 词： | 氟中毒 脑组织 学习记忆能力 |
Changed intelligence of rats with coal-burning fluorosis and its correlation with expression of brain ERK1/2 signal transduction |
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| RAN Long-yan,GUI Chuan-zhi,WU Chang-xue,et al.. Changed intelligence of rats with coal-burning fluorosis and its correlation with expression of brain ERK1/2 signal transduction[J]. Chinese Journal of Public Health, 2010, 26(10) |
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| Authors: | RAN Long-yan GUI Chuan-zhi WU Chang-xue et al. |
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| Affiliation: | RAN Long-yan,GUI Chuan-zhi,WU Chang-xue,et al.Department of Pathology and Molecular Biology,Guiyang Medical College(Guiyang 550004,China) |
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| Abstract: | Objective To investigate the impairment of learning and memory ability in rats with coal-buring fluorosis and its mechanism.Methods The rat model of coal-burning fluorosis was created by feeding the animals with the corn polluted by fluoride during corn-drying processe.The behavior changes of the rats were tested by Morris water maze test and ERK1/2 expression in brain tissue was assessed with Western blotting or real-time PCR.Results In Morris water maze test,the rats with fluorosis exhibited a longer esca... |
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| Keywords: | ERK1/2 |
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