人巨细胞病毒激活感染与颈动脉粥样硬化斑块不稳定性的临床研究

目的探讨人巨细胞病毒（HCMV）激活感染与颈动脉粥样硬化的关系。方法采用免疫荧光法检测外周血自细HCMV—PP65抗原作为HCMV激活感染的指标，对212例颈动脉粥样硬化患者分别进行HCMV—PP65抗原、血清基质金属蛋白酶-9（MMP-9）、肿瘤坏死因子-α（TNF—α）水平检测和颈动脉超声检查。结果HCMV—PP65抗原阳性组、阴性组血清MMP-9含量分别为（242．25±81．03）、（159．57±79．20）pg／mL，差异有统计学意义（P〈O．01）；两组TNF—α含量分别为（3．04±0．37）、（1．37±0．17）ng／mL，差异有统计学意义（P〈O．01）；HCMV—PP65抗原阳性组44例（69．84％）存在不稳定性斑块，HCMV—PP65抗原阴性组72例（48．32％）存在不稳定性斑块，差异有统计学意义（x^2=8．82，P〈0．01）。结论人巨细胞病毒激活感染可引起MMP-9、TNF—α水平增高，可能与颈动脉粥样硬化斑块不稳定性有关。

Human cytomegalovirus activate infection concerned with carotid atherosclerosis

Objective To study the human cytomegalovirus activate infection concerned with carotid atherosclerosis. Methods HCMV-PP65 in peripheral blood leukocytes, which was used as an indicator of active HCMV infection, was detected by immunofluorescence. A total of 212 patients with carotid atherosclerosis was carried out to check HCMV-PP65 antigen level of MMP-9 and TNF-α and carotid ultrasonography. Results Contents of MMP-9 and TNF-α in two group difference were （242.25±81.03）, （159.57±79.20） pg/mL and （3.04±0.37）, （1.37±0.17）ng/mL, there were significant difference between 2 groups （P〈0.01, P〈0.01）. The positive and negative of HCMV-PP65 antigenaemia with destabilization of carotid plaque were 69.84%, 48.32% respectively. Statistically, there was significant difference of HCMV-PP65 antigenaemia between the two groups （x^2=8.82, P〈0.01）. Conclusion Activate infection of HCMV may induce the raising of level of MMP-9 and TNF-±α. It may be associated with the instability of carotid atherosclerosis plaque will be more.