| 梗阻性肾病中肾小管周围毛细血管损伤的实验研究 |
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| 引用本文： | 樊彩斌,温端改,侯建全,郭震华,周云,严向明,王兴东.梗阻性肾病中肾小管周围毛细血管损伤的实验研究[J].江苏医药,2008,34(4):386-389. |
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| 作者姓名： | 樊彩斌 温端改 侯建全 郭震华 周云 严向明 王兴东 |
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| 作者单位： | 1. 苏州大学附属第一医院泌尿外科,215006
2. 苏州大学附属儿童医院泌尿外科 |
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| 摘 要： | 目的 观察梗阻性肾病模型中肾小管周围毛细血管(PTC)损伤与肾小管上皮细胞凋亡的关系,并探讨血管内皮细胞生长因子(VEGF)对PTC的调节作用.方法 建立SD大鼠单侧输尿管完全梗阻(CUUO)模型,用免疫组化方法检测PTC的密度和VEGF的表达水平,TUNEL法对PTC和肾小管上皮细胞进行原位凋亡测定,透射电镜观察超微结构变化.结果 第7天VEGF染色局部增强,PTC数量变化不显著,肾小管上皮细胞凋亡很少见.第14～28天,VEGF表达逐渐下降,直至完全消失.PTC数量减少,肾小管扩张或萎缩明显.电镜显示肾小管上皮细胞、PTC内皮细胞死亡的主要形式为凋亡;TUNEL显示肾小管上皮细胞凋亡在第14天达到高峰,然后迅速下降.结论 PTC减少是肾小管上皮细胞内VEGF表达不足引起的,在肾小管上皮细胞凋亡的过程中发挥了重要作用.
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| 关 键 词： | 梗阻性肾病 肾小管周围毛细血管 血管内皮细胞生长因子 凋亡 |
| 文章编号： | 0253-3685(2008)04-0386-03 |
| 修稿时间： | 2007年6月6日 |
Peritubular capillary injury in experimental obstructive uropathy |
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| FAN Caibin,WEN Duangai,ZHOU Yun,et al..Peritubular capillary injury in experimental obstructive uropathy[J].Jiangsu Medical Journal,2008,34(4):386-389. |
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| Authors: | FAN Caibin WEN Duangai ZHOU Yun |
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| Institution: | FAN Caibin,WEN Duangai,ZHOU Yun,et al.Department of Urology,Affiliated First Hospital,Soochow University,Suzhou 215006,CHINA |
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| Abstract: | Objective Toexplore the relationships between PTC regression and renal tubular cell apoptosis,and investigate PTC regulation by vascular endothelial growth factor (VEGF) during the course of experimental obstructive uropathy. Methods Complete unilateral ureteral obstruction was induced by ligation of the left ureter in SD rats. PTC density and the expression of VEGF and its receptor Flk-1 were detected immunohistochemically, and morphologic changes in PTC endothelial cells and renal tubular epithetial cells... |
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| Keywords: | Obstructive uropathy Peritubular capillary Vascular endothelial growth factor Apoptosis |
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