白念珠菌阴道炎鼠模型构建的研究

目的:不同条件下构建小鼠白念珠菌性阴道炎模型,为白念珠菌性阴道炎发病机制和防治研究提供平台。方法:(1)用同龄小鼠建立雌激素化,免疫抑制和正常对照鼠模型,观察不同时间点各模型小鼠阴道灌洗液真菌载量及阴道组织病理学变化。(2)再对雌激素化组中已自愈的小鼠进行再感染,观察再感染小鼠与初次感染同龄小鼠的真菌载量及阴道组织病理学的变化。结果:雌激素化组、免疫抑制组小鼠自第2天起阴道灌洗液真菌载量水平较高并持续至观察期末,与正常对照组比较前者P<0.05,后者P<0.01,而且从第4天起免疫抑制组明显高于雌激素化组(P<0.05);初发、复发组均高于正常对照组(P<0.05),初发与复发组之间差异无显著性(P>0.05)。阴道组织病理显示雌激素化、免疫抑制及复发感染小鼠在接种后第2天开始至21天,阴道黏膜可见芽生孢子或菌丝侵入,伴有较多的炎性细胞浸润。结论:几种条件下白念珠菌性阴道炎鼠模型构建是成功的,为念珠菌性阴道炎发病机制及防治的研究提供了较理想的平台。

Establishment of vaginal candidiasis murine model under different conditions

Objective: To establish a murine model of vaginal candidiasis under different conditions in order to provide a platform for the study on the pathogenesis, prophylaxis and treatment of vaginal candidiasis. Methods: (1) Three model groups (estrogen-treated group, immunoinhibitor-treated group and normal control group) were established. The number of fungi in vaginal lavage fluid was counted and vaginal pathological manifestations were observed at different time point. (2) Following the spontaneous resolution of the primary infection, the models were reinfected and the amount of fungi and the pathological changes were reassessed. Results: The average level of fungi amount in estrogen-treated group and immunosuppressed group rose from day 2 after the infection and persisted to the end of the observation period (P<0.05, P<0.01). From day 4 after infection, the average level of fungi amount was higher in immunosuppressed group than that in estrogen-treated group (P<0.05). The level of fungi amount in the two above-mentioned groups was higher than that in the control group at each time point (P< 0.05). There was no statistic difference (P>0.05) in the average level of fungi amount between the reinfected group and primary infection. Under microscope it was showed that all vaginal mucosa were invaded by blastospore and hyphe, accompanied with inflammatory cell infiltration, except for control group. Conclusion: The establishment of vaginal candidiasis murine model under different conditions is successful and it provides a platform for the study on the pathogenesis, prophylaxis and treatment of vaginal candidasis.