A sensitive radioimmunoassay of human immunodeficiency virus type-1 p24 antigen in serum |
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| Affiliation: | 1. Chef du service du laboratoire de production des trousses, radio-immunologique commissariat général à l''énergie atomique (CGEA), centre régional d''études nucléaires de Kinshasa (CREN-K), campus universitaires de Kinshasa, BP 868, Kinshasa XI, République démocratique du Congo;2. Faculté de médecine, université de Kinshasa, CGEA/CREN-K BP 868, Kin XI, Kinshasa, République démocratique du Congo;3. Département d''anesthésie et réanimation, clinique universitaire de Kinshasa, CGEA/CREN-K, BP 868, Kin XI, Kinshasa, République démocratique du Congo;1. Key Laboratory for Advanced Materials, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237, PR China;2. State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, East China University of Science and Technology, Shanghai 200237, PR China;1. State Key Laboratory of Food Science and Technology, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047, People''s Republic of China;2. Jiangxi-OAI Joint Research Institute, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047, People''s Republic of China;3. Department of Entomology and UCD Comprehensive Cancer Center, University of California, Davis, CA 95616, USA;4. College of Food Science and Technology, Hainan University, No. 58 Renmin Avenue, Haikou 570228, China;1. Department of Nephrology, Faculty of Medical Sciences, University of Fukui, Fukui, Japan;2. Life Science Innovation Center, University of Fukui, Fukui, Japan;3. Diagnostic System Department, Toyobo Co., Ltd., Osaka, Japan;4. Tsuruga Institute of Biotechnology, Toyobo Co., Ltd., Osaka, Japan;5. Department of Nephrology, Amagasaki Eijinkai Clinic, Hyogo, Japan;6. Department of Nephrology and Dialysis, Kitano Hospital, Tazuke Kofukai Medical Research Institute, Osaka, Japan;7. Department of Internal Medicine, Sugita Genpaku Memorial Obama Municipal Hospital, Fukui, Japan;8. Department of Biological Responses, Institute for Virus Research, Graduate School of Medicine, Kyoto University, Kyoto, Japan |
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| Abstract: | We describe a sensitive and specific radioimmunoassay (RIA) for HIV-1 p24. The test called cgeacren-p24-VIH RIA is a transposition of signal-amplification-boosted of head-denatured plasma ELISA developed by Schupbach to a RIA performed with in-house reagents. This modified assay is based on the use radiolabeled tracer to decrease the lower limit of p24 antigen detection from 0.5 pg/ml [JCM, 2003, 1016–1022] to 0.05 pg/ml by RIA method. The RIA technique is based on the competitive binding of the serum HIV-1 p24 and radiolabeled p24 preparation. They compete for binding to an antibody specific present in a substoichiometric amount. Complex of radiolabeled and unlabeled p24 antigen were separated from unbound species by reaction with an anti-rabbit sheep immunoglobulin as second antibody [10]. This paper describes, for the first time, the successful production of polyclonal anti sera specific for HIV-1 p24 and the development of a sensitive RIA for measuring HIV-1 p24 in serum. The second part of this study will concern the clinical evaluation of this “cgeacren-HIV-1 p24RIA” kit. |
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