The A427 anchorage-independence assay as a screening tool to identify potential chemopreventive agents |
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| Authors: | Sharma Sheela Wyatt Gail P. Elmore Eugene L. Steele Vernon E. |
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| Affiliation: | (1) Cellular and Molecular Toxicology Program, ManTech Environmental Technology Inc., Research Triangle Park, North Carolina, USA;(2) Hematology-Oncology, University of California, Irvine, California, USA;(3) Chemoprevention Branch, Division of Cancer Prevention and Control, National Cancer Institute, Bethesda, Maryland, USA |
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| Abstract: | An in vitro model for screening potential chemopreventive agents using inhibition of anchorage-independent growth of a human lung tumor cell line, A427, is described. A427 cells were selected for the model development, as they are known to be tumorigenic in animals, can grow in soft agarose, and their growth can be inhibited by a well-known chemopreventive agent, 13-cis-retinoic acid. Cells are plated on agarose, allowed to develop colonies for 28 days, the stained colonies are enumerated, and the inhibition of spontaneous colony formation measured. A cytotoxicity test is used concurrently with anchorage independent assay for measuring the relative survival of cells to ensure that any observed inhibition of anchorage independent growth is due to the biological activity of the chemopreventive agents, as it uses human cells as substrates rendering the efficacy data feasible for direct extrapolation to humans. |
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| Keywords: | Anchorage-independent growth Chemoprevention Human lung tumor cells In vitro model |
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