基质金属蛋白酶MMP-2及MMP-9在肺组织损伤修复中的表达

目的观察MMP-2和MMP-9在肺组织损伤修复过程中的表达和变化规律,并探讨它们在肺组织损伤修复中作用的分子机制和意义。方法20只Wister大白鼠,随机分为一个正常组和臭氧攻击损伤后不同时间的实验组,制作大鼠肺损伤模型。应用免疫组化法和图像分析技术观察各组MMP-2及MMP-9蛋白表达的强度。结果成功复制大鼠肺组织损伤动物模型;实验观察表明,MMP-2、MMP-9蛋白表达在正常肺组织为弱阳性,在肺组织损伤时,表达上调,且随着肺组织损伤修复行进而先逐渐增强(<0.05),继而逐渐减弱(<0.05),当肺组织损伤修复达正常时,MMP-2、MMP-9表达恢复到正常水平(>0.05)。结论MMP-2、MMP-9的高表达与肺组织损伤密切相关。

Expression of MMP-2 and MMP-9 in course of injury and repair of lung tissue

Objective: To explore the changes and the expression of MMP-2, MMP-9, MMP-2 mRNA and MMP-9 mRNA in the course of injury and repair of lung tissue, and their significance and the probable molecular mechanism as well. Methods: To establish the injurious model of lung tissue in rats, 20 rats were divided into one control group and eight experimental groups receiving O3 manipulation, and the changes of pathological morphology in different phases were observed under optical microscope. Immunohistochemistry for the expressions of MMP-2 and MMP-9 was performed. Results: The model of rat lung tissue injury was made successfully. Immunohistochemical analysis indicated that the expressions of MMP-2 and MMP-9 were weakly positive in normal lung tissue and positive expression became stronger in injurious lung tissue and their expressions reached the normal level when the lung tissue was repaired. Conclusion: The expressions of MMP-2 and MMP-9 are closely related to the injury of lung tissue. MMP-2 and MMP-9 take part in the whole course of repair and injury of the lung tissue.