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正交试验优选黄秋葵总黄酮提取工艺的研究
引用本文:李南奇,孙炜,肖锋,王慧铭. 正交试验优选黄秋葵总黄酮提取工艺的研究[J]. 广西中医学院学报, 2013, 16(2): 74-76
作者姓名:李南奇  孙炜  肖锋  王慧铭
作者单位:1. 浙江中医药大学,浙江杭州,310053
2. 浙江省中医药学会,浙江杭州,310003
基金项目:浙江省科技厅2010年重大科技专项(项目编号:2010C12013)
摘    要:[目的]研究黄秋葵总黄酮提取的最佳工艺。[方法]以总黄酮得率为考察指标,采用正交试验设计方法,研究乙醇浓度、料液比、提取时间、提取温度4个因素对提取工艺的影响。[结果]影响黄秋葵总黄酮提取的因素依次为:乙醇浓度〉提取温度〉溶媒倍数〉提取时间;结合生产实际,筛选出最佳提取工艺为A1B1C2D3,即乙醇浓度55%、料液比1∶20、提取温度90℃、提取时间1.5 h。在此条件下黄秋葵总黄酮得率为1.79%。[结论]该提取工艺简便、易行、稳定、快捷,为进一步研究、开发黄秋葵资源提供技术手段。

关 键 词:黄秋葵  总黄酮  正交试验  提取

On Extraction Techniques of Total Flavonoids From Abelmoschus Esulentus L.by Orthogonal Test
LI Nan-qi , SUN Wei , XIAO Feng , WANG Hui-ming. On Extraction Techniques of Total Flavonoids From Abelmoschus Esulentus L.by Orthogonal Test[J]. Journal of Guangxi Traditional Chinese Medical University, 2013, 16(2): 74-76
Authors:LI Nan-qi    SUN Wei    XIAO Feng    WANG Hui-ming
Affiliation:1.Zhejiang University of Traditional Chinese Medicine,Hangzhou 310053,Zhejiang,China;2.Zhejiang Association of Chinese Medicine,Hangzhou 310003,Zhejiang,China)
Abstract:[Objective]To investigate the best extraction technique of total flavones from Abelmoschus esulentus L.[Methods] Using orthogonal test,the influences of four factors,ethanol concentration,solid-liquid ratio,extraction time and temperature,on the yield of total flavones were studied.[Results] The most influential factor was ethanol concentration,followed by extraction temperature,solvent quantity and extraction time.The optimum extraction process was A1B1C2D3,which was ethanol concentration of 55%,ratio of solid-liquid 1∶20,extracting temperature of 90 ℃,extracting time of 1.5 h.Total flavones yield was 1.79% under the optimum extraction conditions[.Conclusion]This extraction process is easy,practical,stable and efficient,which provides scientific evidence for research and development of Abelmoschus esulentus L.
Keywords:Hibiscus esulentus L.  total flavones  orthogonal test  extraction
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