正交试验优选黄秋葵总黄酮提取工艺的研究

目的]研究黄秋葵总黄酮提取的最佳工艺。方法]以总黄酮得率为考察指标,采用正交试验设计方法,研究乙醇浓度、料液比、提取时间、提取温度4个因素对提取工艺的影响。结果]影响黄秋葵总黄酮提取的因素依次为：乙醇浓度〉提取温度〉溶媒倍数〉提取时间;结合生产实际,筛选出最佳提取工艺为A1B1C2D3,即乙醇浓度55%、料液比1∶20、提取温度90℃、提取时间1.5 h。在此条件下黄秋葵总黄酮得率为1.79%。结论]该提取工艺简便、易行、稳定、快捷,为进一步研究、开发黄秋葵资源提供技术手段。

On Extraction Techniques of Total Flavonoids From Abelmoschus Esulentus L.by Orthogonal Test

Objective]To investigate the best extraction technique of total flavones from Abelmoschus esulentus L.Methods] Using orthogonal test,the influences of four factors,ethanol concentration,solid-liquid ratio,extraction time and temperature,on the yield of total flavones were studied.Results] The most influential factor was ethanol concentration,followed by extraction temperature,solvent quantity and extraction time.The optimum extraction process was A1B1C2D3,which was ethanol concentration of 55%,ratio of solid-liquid 1∶20,extracting temperature of 90 ℃,extracting time of 1.5 h.Total flavones yield was 1.79% under the optimum extraction conditions.Conclusion]This extraction process is easy,practical,stable and efficient,which provides scientific evidence for research and development of Abelmoschus esulentus L.