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| HPLC法测定玉郎伞中水黄皮素的含量 | |
| 引用本文： | 李霄,田辉,王露瑶,李玲.HPLC法测定玉郎伞中水黄皮素的含量[J].广西中医学院学报,2013,16(2):76-78. |
| 作者姓名： | 李霄 田辉 王露瑶 李玲 |
| 作者单位： | 1. 广西康华药业有限责任公司,广西南宁,530007 2. 广西中医药大学,广西南宁,530001 3. 右江民族医学院,广西百色,533000 4. 柳州食品药品检验所,广西柳州,545001 |
| 摘 要： | 目的]建立高效液相色谱法测定玉郎伞中水黄皮素含量的方法。方法]以CLC-ODS（6.0 mm×150 mm,5μm）为色谱柱,0.05 mol/L庚烷磺酸钠–0.05 mol/L磷酸二氢钾（3∶5）]–乙腈（1∶1）用磷酸调pH 3.0为流动相,流速1.0 ml/min,检测波长261 nm,柱温30℃。结果]水黄皮素的线性范围为0.099～0.998μg,平均回收率为100.3%（RSD=1.2%）。结论]所建立的HPLC法可用于玉郎伞中水黄皮素含量测定及质量控制。 |
| 关 键 词： | 高效液相色谱法 玉郎伞 水黄皮素 |
Determination of Karanjin in Millettia pulchra Kurz var.Laxior(Dunn) Z.Wei by HPLC |
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| LI Xiao , TIAN Hui , WANG Lu-yao , LI Ling.Determination of Karanjin in Millettia pulchra Kurz var.Laxior(Dunn) Z.Wei by HPLC[J].Journal of Guangxi Traditional Chinese Medical University,2013,16(2):76-78. | |
| Authors: | LI Xiao TIAN Hui WANG Lu-yao LI Ling |
| Institution: | 1.Guangxi Konwa Pharmaceuticals Co.Ltd.,Nanning 530007,Guangxi,China;2.Guangxi University of Chinese Medicine,Nanning 530001,Guangxi,China;3.Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China;4.Liuzhou Institute for Food and Drug Control,Liuzhou 545001,Guangxi,China) |
| Abstract: | Objective]To establish HPLC method for determination of karanjin in Millettia pulchra Kurz var.Laxio（r Dunn）Z.Wei.Methods]HPLC was conducted on CLC-ODS（6.0 mm ×150 mm,5 μm）column.The mobile phase was 0.05 mol/L sodium heptanesulphonate-0.05 mol/L potassium dihydrogen phosphate（3∶5）]– acetonitrile（1∶1）,with pH 3.0 through phosphoric acid.The flow rate was 1.0 ml/min.The detection wavelength was 261 nm,and column temperature was set at 30 ℃.Results]Through HPLC analysis,the linear range of karanjin was 0.099~0.998μg,and the average recovery was 100.3% and RSD was 1.2%.Conclusion]The HPLC established can be used for determination and quality control of karabjin in Millettia pulchra Kurz var.Laxio（r Dunn）Z.Wei. |
| Keywords: | HPLC Millettia pulchra Kurz var Laxio（r Dunn）Z Wei karabjin |
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