| 四种蝇的随机序列扩增多态性分析 |
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| 引用本文： | 赵瑞君,尹镭,乔中东,李国锦,段国荣,梁爱华,乔忠红. 四种蝇的随机序列扩增多态性分析[J]. 寄生虫病与感染性疾病, 1996, 0(1) |
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| 作者姓名： | 赵瑞君 尹镭 乔中东 李国锦 段国荣 梁爱华 乔忠红 |
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| 作者单位： | 山西医学院，山西大学生命科学系，厦门大学生物系 |
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| 基金项目： | 山西省归国留学人员基金 |
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| 摘 要： | ＲＡＰＤ技术是在１９９０年后发展起来的一项分子生物学手段，它被越来越广泛地应用在昆虫分类方面。它有许多优越性，如简便、快捷、灵敏度强、经济、对材料要求不高等。更重要的是，它可以从分子的角度出发揭示ＤＮＡ的多态性，从而把物种区分开来。本文以家蝇，丝光绿蝇，尾黑麻绳和夏厕蝇为对象，用３４种１０ｈｐ引物对上述四种蝇的ＤＮＡ进行了地毯式的扩增，以筛选能够用于种类区别的引物。我们的结果显示，引物ＯＰＨ－８可以作为科间的鉴别依据，另有１４种引物可用于蝇科内各属间或种间的分类依据。
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| 关 键 词： | DNA，随机序列扩增多态性，蝇类分类 |
FOUR SPECIES OF FLY DIFFERENTIATION BY RAPD-PCR |
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| Zhao Ruijun et al.. FOUR SPECIES OF FLY DIFFERENTIATION BY RAPD-PCR[J]. Parastoses and Infectious Diseases, 1996, 0(1) |
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| Authors: | Zhao Ruijun et al. |
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| Abstract: | This paper is an application of RAPD-PCR (random amplified poiymorphic DNA polymerase chain reaction) technique in fly taxonomy. Four species of fly from three family were used in screening the 34 random primers of to base in order to identify the fly groups from each other. The results showed that only one primer(OPH-8) could classify the four species and could be used as family key primer. As well as another 14 primers could grouped the family Muscidae. The authors concluded that RAPD method can be used in the entomological construction of diagnostic profiles for species or groups identification and differentitation among conspecific populations. |
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| Keywords: | Random amplified polymorphic DNA (RAPD) PCR fly taxonomy |
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