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| 蚯蚓体腔液体外抗肿瘤作用的实验研究 | |
| 引用本文： | 王建华,关涛,王晓媛,刘志贞,张建林,王秀伟,王国亮,钟儒刚,牛勃.蚯蚓体腔液体外抗肿瘤作用的实验研究[J].中华实验和临床病毒学杂志,2010,24(6):409-411. |
| 作者姓名： | 王建华 关涛 王晓媛 刘志贞 张建林 王秀伟 王国亮 钟儒刚 牛勃 |
| 作者单位： | 1. 北京工业大学生命科学与生物工程学院,北京,100022 2. 山西医科大学生物化学与分子生物学教研室 3. 首都儿科研究所生物技术室 |
| 摘 要： | 目的 探讨蚯蚓体腔液（Earthworm coelomic fluid,ECF）对肿瘤细胞株Siha,SW480,Colo205和PC12的生长抑制作用及作用机制.方法 通过MTT法、流式细胞仪检测及形态学观察分析蚯蚓体腔液粗提物对肿瘤细胞株及正常细胞株（Cos7）的增殖抑制作用.结果 ECF对Siha、SW480、Colo205、PC12细胞的生长均有抑制作用,但对不同肿瘤细胞系的敏感度不同.结论 蚯蚓体腔液粗提物在体外通过诱导肿瘤细胞凋亡有明显的抗肿瘤作用. |
| 关 键 词： | 寡毛目 抗肿瘤药 细胞系 |
Study on antineoplastic effect of earthworm coelomic fluid in vitro |
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| WANG Jian-hua,GUAN Tao,WANG Xiao-yuan,LIU Zhi-zhen,ZHANG Jian-lin,WANG Xiu-wei,WANG Guo-liang,ZHONG Ru-gang,NIU Bo.Study on antineoplastic effect of earthworm coelomic fluid in vitro[J].Chinese Journal of Experimental and Clinical Virology,2010,24(6):409-411. | |
| Authors: | WANG Jian-hua GUAN Tao WANG Xiao-yuan LIU Zhi-zhen ZHANG Jian-lin WANG Xiu-wei WANG Guo-liang ZHONG Ru-gang NIU Bo |
| Institution: | 1.Department of Life Sciences and Bioengineering, Beijing University of Technology, Beijing,100022, China;) |
| Abstract: | Objective To explore the antineoplastic effect in vitro of earthworm coelomic fluid (ECF) on growth inhibition and its mechanism for the tumor cell lines Siha, SW480, Colo205 and PC12.Methods MTT colorimetric assay, flow cytometry and morphological ananlysis were used to test its antitumor activity on tumor cell lines and normal cell line Cos7 in vitro. Results ECF can inhibit the cell growth of Siha, SW480, Colo205, PC12 and Cos7. But different tumor cell lines showed different sensitivity. Conclusion EFC can significantly inhibit the proliferation of tumor cells in vitro by inducing tumor cells apoptosis. |
| Keywords: | Oligochaeta Antineoplasticagents Cell line |
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