| 军团菌5SrRNA聚合酶链反应方法的建立及应用 |
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| 引用本文： | 白羽, 孔繁学, 郭军巧, 孙立华, 邱琼. 军团菌5SrRNA聚合酶链反应方法的建立及应用[J]. 中国公共卫生, 2000, 16(5): 412-413. DOI: 10.11847/zgggws2000-16-05-21 |
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| 作者姓名： | 白羽 孔繁学 郭军巧 孙立华 邱琼 |
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| 作者单位： | 1.辽宁省卫生防疫站 沈阳110005;2.中国预防医学科学院流行病学微生物学研究所 |
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| 摘 要： | 建立5SrRNA基因检测军团菌的多聚酶链反应方法,扩增参考菌株的染色体DNA(L.Pneumophila1～14、L.bozemanni、L.dumoffii、L.longbeachae、L.micdadei、L.jordanis),均可检出104bp的基因片段,敏感性为8.52*102cfu/ml(平均值);而扩增9株非军团菌均为阴性。对模似血标本的检测,其敏感性可达103cfu/ml.检测17例血标本,阳性率为58.8%(10/17),与血清学检测和临床诊断治疗结果相符合。结果表明该方法敏感、特异、快速、简便。
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| 关 键 词： | 5SrRNA基因 军团菌 聚合酶链反应 |
| 收稿时间： | 2000-01-13 |
Establishment and Application of a 5SrRNA Polymerase Chain Reaction Assay for Detection of Legionella Species |
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| Bai Yu, . Establishment and Application of a 5SrRNA Polymerase Chain Reaction Assay for Detection of Legionella Species[J]. Chinese Journal of Public Health, 2000, 16(5): 412-413. DOI: 10.11847/zgggws2000-16-05-21 |
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| Authors: | Bai Yu |
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| Affiliation: | 1.Liaoning Provine Publical Health and Anti epidemic Station, 110005 |
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| Abstract: | A 5SrRNA Polymerase Chain reaction(PCR)assays for the detection of Legionella Species was established.A 104bp fragment of the 5SrRNA gene could be detected by amplifing genetic DNA of reference strains(Lp1～14、Lb、Ld、Ll、Lm、Lj).9 non legionella strains could not produce a positive amplified fragment.The sensitive for reference strains and blood were 8.5*102cfu/ml(average)and 102cfu/ml respectively.This PCR system has been successful in detecting 17 clinical samples,the positive rate were 58.8% (10/17).Above mentioned result showed that this assay was sensitive,rapid,specific and easy to perform. |
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| Keywords: | SrRNA gene Legionella Polymerase chain reactin(PCR) |
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