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Apoptotic cell death and its relationship to gastric carcinogenesis |
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| Authors: | Bir Ferda Calli-Demirkan Nese Tufan A Cevik Akbulut Metin Satiroglu-Tufan N Lale |
| Institution: | 1. Pamukkale University, School of Medicine, Department of Pathology,Denizli, Turkey 2. Pamukkale University, School of Medicine,Department of Histology and Embryology, Denizli, Turkey 3. Pamukkale University, School of Medicine, Department of Medical Biology, Center for Genetic Diagnosis, Molecular Genetics Laboratory, Denizli, Turkey |
| Abstract: | AIM: To investigate the apoptotic process of cells within the intestinal metaplasia areas co-localizing with chronic gastritis and gastric carcinomas and to analyze the involvement of proteins regulating apoptosis in the process of intestinal metaplasia related gastric carcinogenesis. METHODS: Forty-two gastric carcinoma and seventeen chronic gastritis cases were included in this study. All cases were examined for the existence of intestinal metaplasia. Ten cases randomly selected from each group were processed for TUNEL assay. TUNEL positive cells within the intestinal metaplasia areas, co-localizing either to gastric carcinoma or chronic gastritis, were counted and converted to apoptotic indices. In addition, p53, bcl-2 and bax expression patterns within these tissues were analyzed on the basis of immunohistochemistry. RESULTS: Twenty-eight of the cases were intestinal and 14 of the cases were diffuse type adenocarcinomas. 64% (27/42) of the gastric carcinoma cases had intestinal metaplasia. Intestinal metaplasia co-localized more with intestinal type carcinomas compared with diffuse type carcinomas 75% (21/28) vs 42% (6/14), respectively; P |
| Keywords: | p53 Bax Bcl-2 TUNEL staining Intestinal metaplasia Apoptosis Gastric cancer |
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