| 石蜡包埋组织的p16基因甲基化特异性PCR技术 |
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| 引用本文： | 颜召文,张帆,吴平平,赵雁飞. 石蜡包埋组织的p16基因甲基化特异性PCR技术[J]. 上海交通大学学报(医学版), 2003, 23(3): 231-233 |
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| 作者姓名： | 颜召文 张帆 吴平平 赵雁飞 |
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| 作者单位： | 上海第二医科大学病理教研室，上海第二医科大学病理教研室，上海第二医科大学病理教研室，上海第二医科大学病理教研室 上海 200025，上海 200025，上海 200025，上海 200025 |
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| 摘 要： | 目的：以石蜡包埋组织为材料进行DNA甲基化特异性PCR(MSP)检测，研究其可行性。方法：40例食管组织以中性福尔马林固定、包埋、脱蜡、DNA样品制备和亚硫酸盐修饰后进行PCR测定。2例MSP阳性的冰冻甲状腺组织以70％酒精、丙酮和中性福尔马林分别固定及石蜡包埋后，非甲基化引物PCR扩增，比较不同固定剂的MSP效果。结果l石蜡包埋食管组织MSP检出率与文献报道的冰冻组织MSP检出率相同，三种常用方法固定的甲状腺组织均能获得理想的MSP效果。结论：MSP通过选择合适的实验条件，可应用于石蜡包埋组织。
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| 关 键 词： | 石蜡包埋组织 p16基因 DNA甲基化 PCR 基因失表达 样品制备 |
| 文章编号： | 0258-5898(2003)03-0231-03 |
| 修稿时间： | 2002-09-25 |
Methylation-Specific Polymerase Chain Reaction of p16 Gene on the Parafin-Embedded Tissues |
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| VAN Zhao-wen,ZHANG Fan,WU Ping-ping,et al. Methylation-Specific Polymerase Chain Reaction of p16 Gene on the Parafin-Embedded Tissues[J]. Journal of Shanghai Jiaotong University:Medical Science, 2003, 23(3): 231-233 |
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| Authors: | VAN Zhao-wen ZHANG Fan WU Ping-ping et al |
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| Abstract: | Objective To test the methylation-specific polymerase chain reaction (MSP) on the parafin-embedded tissues. Methods Forty esophageal samples were fixed by neutral buffered formalin, then parafin-embedded and detected by MSP; 2 MSP positive thyroid samples were cut into 3 parts and fixed by 70% ethenal, neutral buffered formalin and acetone, then parafin-embedded and detected by MSP. Results There was no significant difference between the parafin-embedded esophageal tissues and the frozen tissues (in reference) on MSP positivity; All three fixation methods had fine outcomes on MSP positive thyroid tissue. Conclusion The MSP methods can be successfully used on parafin-embedded tissues. |
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| Keywords: | parafin -embedded tissues DNA methylation methylation - specific PCR |
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