血管活性物质对人内皮细胞C-型利钠利尿肽合成和释放的影响

目的:在培养的人内皮细胞上观察内皮素(ET)、血管紧张素Ⅱ(AngⅡ)和同型半胱氨酸(Hcy)对C-型利钠利尿肽(CNP)生成和释放的影响,以探讨CNP生成和释放的机制。方法:人内皮细胞培养;CNP放免测定。结果:ET、AngⅡ均可刺激内皮细胞CNP的生成,10^-9、10^-8、10^-7mol/L的ET和AngⅡ分别使细胞CNP含量较对照组高1%(P＞0.05),49%(P＜0.05),117%(P＜0.01)和137%(P＜0.01),165%(P＜0.01),201%(P＜0.01),大剂量ET、AngⅡ可刺激CNP的释放。Hcy对内皮细胞CNP的生成没有影响,但10 ^-9、10^-8、10^-7mol/LHcy可使其释放增加17%(P＞0.05),84%(P＜0.01)和555%(P＜0.01)。结论:ET、Ang和Hcy可调节CNP的释放和/或生成。

The effect of vasoactive substances on synthesis and release of C-type natriuretic peptide in cultured human endothelial cells

AIM: To investigate the effect of endothelin (ET), angiotensin II (AngII) and homocysteine (Hcy) on C-type natriuretic peptide (CNP) synthesis and release. METHODS: Human endothelial cell was cultured; CNP was measured by radioimmunoassay method. RESULTS: ET and AngII could augment CNP synthesis in human endothelial cells. Compared with control group, 10 -9 ,10 -8 ,10 -7 mol/L ET and Ang II increased CNP content of endothelial cells by 1%( P >0.05), 49%( P <0.05),117%( P <0.01) and 137% ( P <0.01),165%( P <0.01),201%( P <0.01),respectively. A great dose of ET and Ang II also stimulated CNP release from cultured human endothelial cells. Hcy had no effect on CNP synthesis, but 10 -9 ,10 -8 ,10 -7 mol/L Hcy enhanced CNP release from cultured human endothelial cells by 17%( P >0.05),84%( P <0.01) and 555%( P <0.01), respectively. CONCLUSION: ET, AngII and Hcy might be involved in the synthesis and release of human endothelial cell CNP.