雷米普利对单侧输尿管梗阻大鼠肾组织BMP-7表达的影响

目的 观察雷米普利对单侧输尿管梗阻（unilateral ureteral obstructionUU0）大鼠肾脏间质损伤及骨形态蛋白-7（bone morphogenetic protein-7 BMP-7）表达的影响。方法3（）只雄性SD大鼠随机分为假手术组（SHAM组），1周模型组（UUO-1组），2周模型组（UU0-2组），1周干预组（ACEF1组），2周干预组（ACEF2组）。各组大鼠于术后相应时间（除UUO-1组为术后1周，其余各组均为术后2周）取梗阻侧肾组织行Masson染色，按小管问质损害的特征进行半定量评分，用RT-PCR方法检测肾组织BMP-7mRNA水平。结果①SHAM组肾间质损伤指数较低，BMP-7高表达于肾组织。UUO-1组与sHAM组比较，肾间质损伤指数增加，BMP-7表达下降，UUO-2组与UUO-1组比较，肾间质损伤指数增加，BMP-7表达下降；②ACEI-2组较UUO-2组肾间质损伤指数下降，BMP-7表达增加；③UUO-1组已有少量间质纤维化，在此基础上ACEF1组比UUO-2组肾间质损伤指数下降，BMP-7表达增加。结论①在肾间质纤维化模型中，随着肾间质纤维化的进展，肾间质损伤指数逐渐增加，BMP-7表达逐渐下降；②雷米普利可部分维持肾间质纤维化过程中BMP-7的表达，从而延缓肾间质纤维化进展，对已发生纤维化的肾组织仍然有此作用。

Effection of Ramipril on expression of renal bone morphogenetic protein-7： an experimental study in unilateral ureteral obstruction rats

Objective To investigate the effect of Ramipril on the expression of bone morphogenetic protein-7 of renal interstitial following unilateral ureteral obstruction. Methods Adult male SD rats were randomly divided into five groups： Sham operated group （ Sham）, UUO vehicle group （UUO-1 and UUO-2）, UUO treated with Ramipril 10 mg·kg^-1·d ^-1） （ACEI-1, ACEI-2）, ACEI-1 group was treated with Ramipril one week after unilateral ureteral obstruction, ACEI-2 group was treated with Ramipril right after unilateral ureteral obstruction. On the 1 week after operation , the obstructed kidneys of UUO-1 group rats were taken out to be measured by Masson stain and m RNA expression for bone morphogenetic protein-7, the other groups were taken out on the 2 week after operation. The degree of tubulointerstitial damage was scored. Results In comparison with UUO-2 group, interstitial expression of bone morphogenetic protein-7 were significantly increased in ACEI-2 group, the degree of tubulointerstitial damage were reduced. On the base of renal tubulointerstitial fibrosis, interstitial expression of bone morphogenetic protein-7 were increased and the degree of tubulointerstitial damage were reduced in ACEI-1 group. Conclusions Ramipril may maintain expression of bone morphogenetic protein-7 on the course of renal tubulointerstitial fibrosis, slow the advance of the tubuointerstitial.