我国2015-2017年急性乙型肝炎病例HBV基因型分布特征分析

目的分析我国急性乙型肝炎(乙肝)病例HBV基因型流行病学分布特征。方法收集2015-2017年中国疾病预防控制信息系统报告的急性乙肝病例620例, 通过巢式PCR扩增获得全长HBV基因组, 构建系统发育树确定HBV基因型, 结合基础资料分析HBV基因型的分布情况。结果在620例急性乙肝病例中, 成功分型519例(83.71%, 519/620), 包括A型(0.19%, 1/519)、B型(27.17%, 141/519)、C型(62.04%, 322/519)、D型(9.06%, 47/519)、I型(0.77%, 4/519)和CD重组型(0.77%, 4/519);2个主要的基因亚型为B2(95.03%, 134/141)和C2(72.67%, 234/322)。基因型在我国7个地区分布不同, C型在东北(94.55%, 52/55)、华北(93.85%, 61/65)、华东(78.87%, 56/71)和华南地区(58.14%, 50/86)的比例较高, B型在华中(58.07%, 36/62)和西南(52.94%, 45/85)地区的比例较高, 西北地区的D型(48.42%,...

Epidemiological distribution of hepatitis B virus genotypes in acute hepatitis B cases in China,2015-2017

Objective To analyze the genotype distribution of acute hepatitis B virus in China. Methods A total of six hundred and twenty acute Hepatitis B cases reported to China Information System for Diseases Control and Prevention from 2015 to 2017 were selected. First, the full-length HBV genome was obtained by nested PCR amplification. In addition, the HBV genotype was determined by constructing a phylogeny tree. Finally, using primarydata, HBV genotype distribution was analyzed. Results A total of 519 (83.71%, 519/620) sequences were obtained genotype of 620 acute hepatitis B cases, including A (0.19%, 1/519), B (27.17%, 141/519), C (62.04%, 322/519), D (9.06%, 47/519), I (0.77%, 4/519) and C/D (0.77%, 4/519); B2(95.03%, 134/141) and C2 (72.67%, 234/322) were the two major subgenotypes. Genotypes were distributed differently in seven regions of China. The proportion of genotype C appeared higher in Northeast China (94.55%, 52/55), North China (93.85%, 61/65), East China (78.87%, 56/71), and South China (58.14%, 50/86). The proportion of genotype B was higher in Central China (58.07%, 36/62) and Southwest China (52.94%, 45/85), the proportion of genotype D was the highest in Northwest China (48.42%, 46/95). A total of 515 cases were classified as serotypes, including ''adr'' (57.48%, 296/515), ''adw'' (30.87%, 159/515), ''ayr'' (0.19%, 1/515), and ''ayw'' (11.46%, 59/515). Genotype B was dominated by ''adw'' serotype (92.14%, 129/140), genotype C was dominated by ''adr'' serotype (91.88%, 294/320),all genotype D were ''ayw'' serotype. The genotype of acute hepatitis B was correlated with serotype, ''adw'' was dominant in genotype B, ''adr'' was dominant in genotype C and ''ayw'' was dominant in genotype D.In different gender and age group, there was no statistical significance ingenotype distribution (P>0.05). Conclusions The genotype of acute hepatitis B in China from 2015 to 2017 was mainly B, C, and D; genotype C was dominant in the Northeast China,North China, East China and South China; B and C were common in Central and Southwest China, and genotype B was dominant. Genotype D was primarily distributed in Northwest China. The genotype of acute hepatitis B was correlated with serotype, ''adw'' was dominant in genotype B, ''adr'' was dominant in genotype C and ''ayw'' was dominant in genotype D. There was no difference in the distribution of acute hepatitis B genotypes among different genders and age groups.