血常规MCHC对标本性状异常的提示价值及抗干扰方案的探讨

目的 探讨血常规平均红细胞血红蛋白浓度(MCHC)水平异常升高的常见干扰原因及抗干扰处理方法.方法 选取2019年8月至2020年6月于该院检验科行血常规检测且首次MCHC水平异常升高(MCHC>380 g/L)的102例患者为研究对象,分析其MCHC水平异常升高的原因.针对不同干扰因素采用水浴、稀释和(或)血浆置换等...

Probing value of blood routine MCHC for abnormal specimen characteristics and discussion on anti-interference solution

Objective To investigate the common interference and anti-interference solution of abnormally elevated mean corpuscular hemoglobin concentration(MCHC)in routine blood test.Methods A total of 102 patients with abnormally elevated MCHC levels(MCHC>380 g/L)who underwent blood routine test in the laboratory department of our hospital from August 2019 to June 2020 were selected as the research objects,and the causes of abnormally elevated MCHC levels were analyzed.According to different interference factors,after treatment by water bath,dilution and/or plasma exchange,the blood routine indicators were retested by automatic blood analyzer.The levels of white blood cell count(WBC),red blood cell count(RBC),hemoglobin(Hb),hematocrit(HCT),mean corpuscular volume(MCV),mean corpuscular hemoglobin(MCH),MCHC and platelet(PLT)before and after treatment were recorded and compared.The indicator levels corrected by different anti-jamming solutions were also compared.Results Among the blood routine specimens of 102 patients,12 cases were lipemia,68 cases were erythrocyte agglutination,20 cases were hemolyzed,and 2 cases were hyperleukocyte(WBC>350×10⁹/L).Compared with before treatment,after plasma exchange treatment of lipid blood samples,there were no significant differences in the levels of WBC and Hb(P>0.05),the levels of RBC and HCT increased,and the levels of MCV,MCH,MCHC and PLT decreased,and the differences were statistically significant(P<0.05).Compared with before treatment,there was no significant difference in the level of each indicator after the hemagglutination specimens were treated with 37℃water bath for 20 min(P>0.05).After being treated in a 37℃water bath for 30 min,the MCHC of 9 specimens could not be corrected,and the MCHC could be corrected accurately after further dilution+incubation(incubating the dilution solution with the specimen)or plasma exchange method.Compared with before treatment,after treatment with 41℃water bath for 10 min,there were significant differences in the levels of other indicators except WBC and Hb(P<0.05).Compared with before treatment,the levels of RBC,HCT,and MCV were increased,and the levels of MCH,MCHC,and PLT were decreased after the hemolyzed specimens were treated with plasma exchange,and the differences were statistically significant(P<0.05).Conclusion For specimens with abnormally elevated levels of MCHC,laboratory personnel should first analyze possible interference factors,select fast and effective methods to correct different interferences,and provide accurate test reports for patients.