| 儿茶素对血管平滑肌细胞的抑制作用及其可能的机制 |
| |
| 引用本文： | 赵锦燕,林薇,洪振丰,叶蕻芝. 儿茶素对血管平滑肌细胞的抑制作用及其可能的机制[J]. 癌变．畸变．突变, 2011, 23(1): 35-37. DOI: 10.3969/j.issn.1004-616x.2011.01.009 |
| |
| 作者姓名： | 赵锦燕 林薇 洪振丰 叶蕻芝 |
| |
| 作者单位： | 福建中医药大学,福建中西医结合研究院,福建福州,350108 |
| |
| 基金项目： | 福建省科技厅重点项目(2009I0013); 福建省自然科学基金项目(2010J01194) |
| |
| 摘 要： | 目的：探讨儿茶素对血管平滑肌细胞生长的抑制作用及其可能的作用机制。方法：采用MTT法检测不同剂量儿茶素对血管平滑肌细胞的抑制作用,分别用50、100、150、200、250、300μg/ml的儿茶素作用血管平滑肌细胞24和48 h后,观察细胞形态学的改变,并用RT-PCR检测儿茶素对血管平滑肌细胞基质金属蛋白酶-2（matrix metalloproteinase-2,MMP-2）和基质金属蛋白酶抑制因子（tissue inhibitor of matrix metalloproteinase-1,TIMP-1）mRNA表达的影响。结果：250μg/ml儿茶素明显抑制细胞的生长,且明显下调血管平滑肌细胞中MMP-2 mRNA的表达,并同时上调TIMP-1 mRNA的表达。结论：儿茶素可以抑制血管平滑肌细胞的生长并且调节MMP-2和TIMP-1 mRNA的表达水平。
|
| 关 键 词： | 儿茶素 血管平滑肌细胞 基质金属蛋白酶-2 金属蛋白酶抑制剂-1 |
Inhibitory effects and possible mechanisms of catechin on vascular smooth muscle cells |
| |
| ZHAO Jin-yan,LIN Wei,HONG Zhen-feng,YE Hong-zhi. Inhibitory effects and possible mechanisms of catechin on vascular smooth muscle cells[J]. Carcinogenesis,Teratogenesis and Mutagenesis, 2011, 23(1): 35-37. DOI: 10.3969/j.issn.1004-616x.2011.01.009 |
| |
| Authors: | ZHAO Jin-yan LIN Wei HONG Zhen-feng YE Hong-zhi |
| |
| Affiliation: | ZHAO Jin-yan,LIN Wei,HONG Zhen-feng,YE Hong-zhi~* (Fujian Academy of Integrative Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350108,Fujian,China) |
| |
| Abstract: | OBJECTIVE:To explore the inhibitory effects of catechin on vascular smooth muscle cells (VSMCs)and its possible mechanisms.METHODS:After VSMCs were exposed to different doses of catechin at 50,100, 150,200,250,300μg/ml,for 24 h and 48 h cell morphology was observed.MTT assay was used to test cell viability, mRNA expressions of MMP-2 and TIMP-1 were detected by RT-PCR analysis.RESULTS:Cell growth was inhibited after exposure to 250μg/ml,and mRNA expression MMP-2 was down-regulated.Meanwhile mRNA expression of TIMP-1 was up-regulated.CONCLUSION:Catechin could inhibit VSMCs and regulate mRNA expressions of MMP-2 and TIMP-1. |
| |
| Keywords: | catechin vascular smooth muscle cells matrix metalloproteinase-2 tissue inhibitor of matrix metalloproteinase-1 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
