| 黄曲霉毒素B1诱导的恶性转化肝细胞miRNA表达谱的变化 |
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| 引用本文： | 李志芳,李道传,王庆,刘彩霞,李文学,陈雯.黄曲霉毒素B1诱导的恶性转化肝细胞miRNA表达谱的变化[J].癌变．畸变．突变,2011,23(1):26-30. |
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| 作者姓名： | 李志芳 李道传 王庆 刘彩霞 李文学 陈雯 |
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| 作者单位： | 中山大学公共卫生学院预防医学系,广东广州,510080 |
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| 基金项目： | 国家自然科学基金项目(30800930); 国家杰出青年基金(30925029) |
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| 摘 要： | 目的：检测黄曲霉毒素B1（aflatoxin B1,AFB1）诱导的恶性转化肝L02细胞（L02T）的miRNA表达谱,寻找差异表达的miRNA。方法：以含有AFB1的培养液多次间歇性染毒L02细胞获得转化细胞L02T,通过miRNA芯片技术检测和分析对照L02和转化L02T细胞的miRNA表达谱;用实时荧光定量PCR方法对芯片结果加以验证;采用TargetScan软件预测miRNA可能调控的靶基因。结果：获得2组细胞856个miRNA的表达谱,在25个表达差异显著的miRNA中,15个表达上调,10个表达下调;用定量RT-PCR对芯片结果中表达差异的miR-320a、miR-638和miR-98进行验证,并对其中上调显著的miR-638进行生物信息学分析,预测到4个与肝癌相关的潜在靶基因。结论：在AFB1诱导的恶性转化肝L02细胞中筛查出25个表达差异显著的miRNA,差异表达的miRNA可能在细胞恶性转化过程中起重要作用。
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| 关 键 词： | 黄曲霉毒素B1 永生化人肝细胞 细胞恶性转化 miRNA |
Differentially expressed miRNA profile of transformed hepatic cells induced by aflatoxin B1 |
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| LI Zhi-fang,LI Dao-chuan,WANG Qing,LIU Cai-xia,LI Wen-xue,CHEN Wen.Differentially expressed miRNA profile of transformed hepatic cells induced by aflatoxin B1[J].Carcinogenesis,Teratogenesis and Mutagenesis,2011,23(1):26-30. |
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| Authors: | LI Zhi-fang LI Dao-chuan WANG Qing LIU Cai-xia LI Wen-xue CHEN Wen |
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| Institution: | LI Zhi-fang,LI Dao-chuan,WANG Qing,LIU Cai-xia,LI Wen-xue,CHEN Wen~* (Faculty of Preventive Medicine,School of Public Health,Sun Yat-sen University,Guangzhou 510080,China) |
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| Abstract: | OBJECTIVE:To identify the differentially expressed miRNAs in malignantly transformed L02 cells(L02T) induced by aflatoxin B1(AFB1),we performed miRNA microarray on both control cells(L02) and L02T cells.METHODS:L02T cells were obtained after exposure to aflatoxin B1(AFB1) several times.The comparison of differential expression profiles between L02 and L02T cells was performed and analyzed.The differentially expressed miRNAs were then selected for validation by semi-quantitative real-time PCR.The targets of ... |
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| Keywords: | aflatoxin B1 immortalized human hepatic cells malignant cell transformation miRNA |
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