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Comparison of the coat protein genes of Lettuce big-vein associated virus isolates from Australia with those of isolates from other continents
Authors:Linda D. Maccarone  Martin J. Barbetti  Krishnapillai Sivasithamparam  Roger A. C. Jones
Affiliation:(1) Faculty of Natural and Agricultural Sciences, School of Plant Biology, University of Western Australia, Stirling Highway, Crawley, WA, 6009, Australia;(2) Department of Agriculture and Food Western Australia, Agricultural Research Western Australia, Locked Bag No. 4, Bentley Delivery Centre, Perth, WA, 6983, Australia;
Abstract:The complete coat protein (CP) nucleotide sequences of seven Lettuce big-vein associated virus (LBVaV) isolates from Australia were compared to those of 22 other LBVaV and five tobacco stunt virus (TStV) isolates. On phylogenetic analysis, clade I contained only LBVaV isolates from Europe, sub-clade IIa only Australian LBVaV isolates, IIb only Japanese LBVaV isolates, and IIc only TStV isolates from Japan. In the amino acid sequences deduced, the central region of the gene was most divergent. Mean Dn/Ds ratios were 0.283 and 0.124 for clades I and II, respectively. The suggestion that TStV is a strain of LBVaV was supported.
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