三七HPLC指纹图谱的建立

目的 :建立三七药材的高效液相指纹图谱 (HPLC-FP) ,在量上为三七的鉴定与合理应用提供依据。方法 :LunaC₁₈(2 )色谱柱 ,流动相 :(A)乙腈 (B) 0.05%磷酸梯度洗脱 ,0～8min(20%A ,80%B) ,8～20min(20%A～40%A ,80%B～60 %B) ,20～ 30min(40 %A～20 %A ,60 %B～80 %B) ,30～36min(20%A～100%A ,80%B～0) ,36～50min (100%A) ,50～65min(100%A～20%A ,0～80%B) ,流速 1.0mL·min^-1 ,检测波长 203nm。咖啡因为内标物。结果 :通过聚类分析 ,21个不同产地、不同规格的三七样品被聚为 4类 :地道药材优级品、地道药材、市售一般品和次品。通过相似度计算 ,21个三七样品的相似度均在0.9～1.0之间 ,8个常见伪品的相似度均小于0.9。结论 :该色谱图可作为三七的HPLC-FP ,用于三七的真伪鉴别和质量评价。

Fingerprint Construction of Panax notoginseng

OBJECTIVE: To provide application and identification basis for Panax notoginseng by constructing the HPLC-FP of Panax Notoginsing. METHOD: The luna C18(2) column was used with a mobile phase of (A) acetonitrile-(B) 0.05% phosphoric acid gradient elution, 0-8 min (20% A, 80% B), 8-20 min (20% A-40% A, 80% B-60% B), 20-30 min(40% A-20% A, 60% B-80% B), 30-36 min(20% A-100% A, 80% B-0), 36-50 min(100% A), 50-65 min (100% A-20% A, 0-80% B). The flow rate was 1.0 ml.min-1. The wavelength of detecter was set at 203 nm. Caffeine was internal standard. RESULT: By Cluster Analysis, the twenty-one notoginseng samples were classified as four clusters: the superior in producing area, the ordinary in producing area, the ordinary and the inferior. By Similarty Calculation, the similarity of the twenty-one notoginseng samples were 0.9-1.0, and the similarity of the eight common falses are less than 0.9. CONCLUSION: The HPLC-FP of notoginseng has been established. The method can be used to identify and evaluate the quality of notoginseng.