大豆异黄酮抗绝经后骨质疏松骨丢失分子机制的初步研究

目的:观察大豆异黄酮(Soybean Isoflavaones)对去卵巢大鼠骨组织中骨保护素(OPG)、破骨细胞分化因子(ODF)和巨噬细胞集落刺激因子(M-CSF)mRNA表达的影响,探讨大豆异黄酮抗绝经后骨质疏松(PMO)骨丢失的可能分子机制.方法:健康3月龄雌性SD大鼠30只,随机平均分为假手术组,去卵巢组和大豆异黄酮组(大豆异黄酮,50mg/kg·d,灌胃).假手术组仅牵动卵巢,其余两组均行卵巢切除术.12周后取第3～6腰椎做骨密度检查;取股骨提总RNA,实时定量PCR(Real-time quantitative PCR)法检测上述因子mRNA的表达情况.结果:大豆异黄酮能够增加去卵巢大鼠腰椎骨密度,并可上调去卵巢大鼠骨组织OPG的mRNA表达,下调M-CSF的mRNA表达,使ODF/OPG值下降.结论:大豆异黄酮抗绝经后骨质疏松骨丢失的分子机制可能与其调控OPG和M-CSF的表达和ODF/OPG值有关.

The Molecule Mechanism of Soybean Isoflavaones Antagonizing Bone Loss Induced by Postmenopausal Osteoporosis

Objective: To observe the effect of the Soybean Isoflavaones on the mRNA expression of Osteoprotegerin(OPG),Osteoclast Differentiation Factor(ODF) and Macrophage Colony stimulating Factor(M-CSF) in bone tissue of ovariectomized rat,and to investigate the possible molecule mechanism of Soybean Isoflavaones antagonizing bone lose induced by postmenopausal osteoporosis.Methods:Thirty healthy adult SD rats were randomly divided into 3 groups:sham-operated group,ovariectomized group and Soybean Isoflavaones treated group.All rats were ovariectomized except those in sham-operated group.Bone density of the 3-6th lumbar vertebrae were detected after 12 weeks.Total RNA were extracted from femur bone and the mRNA expression of OPG,ODF and M-CSF were examined by real time PCR.Results:The data showed that Soybean Isoflavaones increased the bone density of the ovariectomized rat lumbar vertebrae and up-regulated the expression of OPG,whereas down-regulated the expression of M-CSF and the ratio of ODF:OPG.Conclusion:The molecule mechanism of Soybean Isoflavaones antagonizing bone loss induced by postmenopausal osteoporosis is tightly correlated with the regulation of expression of OPG,M-CSF and the ratio of ODF:OPG.