柱前衍生化法评价不同品种和产地柴胡药材和饮片的质量

目的:通过对大量柴胡药材和柴胡饮片样品中柴胡总皂苷和柴胡皂苷a,d的含量进行测定评价不同产地、不同品种柴胡样品的质量优劣。方法:分光光度法测定柴胡总皂苷的含量;柱前衍生化HPLC-UV测定柴胡皂苷a,d的含量。结果:红柴胡、黑柴胡的皂苷类成分较北柴胡低,竹叶柴胡几乎检不出柴胡皂苷;北柴胡的柴胡皂苷a的质量分数主要在0.100%~0.330%,柴胡皂苷d 0.200%~0.400%,柴胡总皂苷1.00%~2.00%;粗根无分支柴胡皂苷成分明显低于细根或粗根多分支柴胡药材。结论:建议北柴胡的柴胡皂苷a的含量不得低于0.100%,柴胡皂苷d不得低于0.200%,柴胡总皂苷不得低于1.00%;北柴胡的适宜产区为山西中部的太谷,山西南部的芮城,平陆,万荣,山西东南部的长治,甘肃的陇西,礼县等地;选育北柴胡品种时优先选取根粗且多分支的品种。

Determination of content of saikoside in Bupleurum Chinense from different sources

Objective: To determine the contents of saikosaponin a,d and saikosaponins in Radix Bupleuri from different and assess their quality.Method: Saikosaponin a and d were used as the chemical reference substance to establish RP-HPLC and visible-spectrophotometry methods for determination of contents of saikosaponin a,d and saikosaponins.Result: The quality of B.chinense was better than that of B.scozonerifolium and B smishii;The contents of a,saikosaponin d and total saikosaponin in B.chinense were 0.100%-0.330%,0.200%-0.400% and 1.00%-2.00%,respectively.Conclusion: It is to suggest that the minimal contents requirements of saikosaponin a,d and saikosaponins in B.chinense should be set at 0.100%,0.200% and 1.00%,respectively;the best suitable planting areas are Taigu in the middle of Shanxi province,Ruicheng,Pinglu and Wanrong in the south of Shanxi province,and Longxi,Lixian in Gansu province.