流行性出血热病毒单克隆抗体在临床早期诊断中应用的研究

用IFAT检测77例临床诊断为EHF病人的外周血白细胞中的病毒抗原。阳性率为50.6％。2—5病日的早期病人阳性率为64.3％(27/42)。并发现,病日越早,病情越重,阳性率越高。试验中应用了抗EHF病毒McAb4B_9、4E_7、3H_4株。抗原阳性者对3种McAb的反应表现为5种类型。不同McAb对抗原的检出率不同,混合使用McAb可提高阳性检出率。在EHF病毒抗原阳性早期患者中,急性期血清特异性IgM抗体阳性率为96.3％。若联合检测EHF病毒抗原和急性期血清IgM抗体,可提高早期诊断的阳性率和准确性。

Applied Study of McAb Against Epidemic Hemorrhagic Fever virus (EHFV) in Early Clinical Diagnosis

Indirect Fluorescent Antibody Test (IFAT) was employed to detect viral antigen in peripheral blood lymphocytes from 77 EHF patients clinically diagnosed. The positive rates were 50.7% and 64.3% (27/42) for early cases (2nd-5th day after onset). It was found that the earlier and more severe the cases were the higher the positive rates. Three McAbs, 4B8, 4E7. 3H4 have been introduced to IFAT. Five reaction patterns of three McAbs with viral antigen positive cases were observed. The McAb showed the difference in positive rates. Combination of McAb could raise the positive rate. In early phase cases with positive EHFV antigens in WBC, the positive rate of specific IgM in acute phase serum was 96.3%. The positive rate and accuracy in the early diagnosis of EHF could be improved by combined application of detection of the viral antigens with acute phase IgM detection.