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| AB-8大孔吸附树脂纯化双黄连中金银花、连翘提取物工艺 | |
| 引用本文： | 李淑芳,王淑美,梁生旺,王战红.AB-8大孔吸附树脂纯化双黄连中金银花、连翘提取物工艺[J].中国实验方剂学杂志,2012,18(2):4-7. |
| 作者姓名： | 李淑芳 王淑美 梁生旺 王战红 |
| 作者单位： | 1. 郑州市第三人民医院,郑州,450000 2. 广东药学院,广州,510000 3. 河南中医学院,郑州,450000 |
| 基金项目： | 国家自然科学基金项目(81073024);广东省自然基金项目(945102240);河南省自然基金项目(0411044800) |
| 摘 要： | 目的:优化AB-8大孔吸附树脂纯化双黄连中金银花、连翘提取物的工艺.方法:以提取物中总酚、总黄酮的含量为考察指标,从树脂径高比、吸附浓度、吸附流速、洗脱剂浓度等方面对大孔吸附树脂纯化工艺进行优化.结果:最佳纯化工艺为金银花、连翘提取物,加蒸馏水稀释成1.5 g·mL-1(生药量),树脂柱径高比1∶10,最大吸附量与树脂体积比为1∶3.3,水洗3倍树脂体积,4BV 70％乙醇洗脱.结论:AB-8大孔吸附树脂纯化金银花、连翘提取物的最佳工艺稳定可行. |
| 关 键 词： | 双黄连 大孔吸附树脂 纯化工艺 |
| 收稿时间： | 2011/8/21 0:00:00 |
Purification Technology of Extracts from Lonicerae japonicae and Forsythiae suspensa in Shuanghuanglian by AB-8 Macroporous Resin |
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| LI Shu-fang,WANG Shu-mei,LIANG Sheng-wang and WANG Zhan-hong.Purification Technology of Extracts from Lonicerae japonicae and Forsythiae suspensa in Shuanghuanglian by AB-8 Macroporous Resin[J].China Journal of Experimental Traditional Medical Formulae,2012,18(2):4-7. | |
| Authors: | LI Shu-fang WANG Shu-mei LIANG Sheng-wang and WANG Zhan-hong |
| Institution: | 1.Third People’s Hospital of Zhengzhou,Zhengzhou 450000,China; 2.Guangdong Pharmaceutical University,Guangzhou 510000,China; 3.Henan University of Traditional Chinese Medicine,Zhengzhou 450000,China) |
| Abstract: | Objective:To optimize purification technology of extracts from L.japonicae and F.suspensa in Shuanghuanglian by AB-8 macroporous resin.Method: The content of total phenolic and total flavonoids were used as indexes.Purification technology was optimized from proportion of diameter-height,adsorption concentration,adsorption speed and eluent concentration,etc.Result: Optimum purification technology was as follows: extracts of L.japonicae and F.suspensa were diluted to concentration was 1.5 g·mL-1by water,ratio of diameter-height 1∶10,proportion of maximum adsorption to resin volume was 1∶3.3,eluted by 3 BV of water,4 BV 70% ethanol as the eluent.Conclusion: This process of purification technology was stable and feasible. |
| Keywords: | Shuanghuanglian macroporous resin purification technology |
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