| 正交试验法优化赤芍总苷闪式提取工艺 |
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| 引用本文： | 刘玉峰,王程程,刘宇,高飞,陈立江. 正交试验法优化赤芍总苷闪式提取工艺[J]. 中国实验方剂学杂志, 2012, 18(2): 12-15 |
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| 作者姓名： | 刘玉峰 王程程 刘宇 高飞 陈立江 |
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| 作者单位： | 辽宁大学药学院,沈阳,110036 |
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| 基金项目： | 辽宁省自然基金项目(20092015);沈阳市科技项目(F10-205-1-28);辽宁大学青年科研基金项目(2009LDQN-KJ-11);辽宁大学"211工程"三期建设-天然产物化学制药项目 |
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| 摘 要： | 目的:优化赤芍总苷闪式提取工艺.方法:利用闪式提取器对赤芍总苷进行提取,HPLC测定含量,并以芍药苷和芍药内酯苷的提取量作为主要指标,采用L9(34)正交试验法,考察总苷闪式提取的最佳工艺.结果:闪式提取赤芍总苷的最佳提取工艺为用30倍量的水浸泡6h,并采用70 V电压提取2 min.结论:闪式提取法可应用于赤芍总苷的提取,该工艺简单,迅速,具有良好的应用前景.
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| 关 键 词： | 赤芍总苷 芍药苷 芍药内酯苷 闪式提取 正交试验 高效液相色谱 |
| 收稿时间： | 2011-09-05 |
Optimization of Smashing Tissue Extraction Process of Total Paeony Glycoside by Orthogonal Test |
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| LIU Yu-feng,WANG Cheng-cheng,LIU Yu,GAO Fei and CHEN Li-jiang. Optimization of Smashing Tissue Extraction Process of Total Paeony Glycoside by Orthogonal Test[J]. China Journal of Experimental Traditional Medical Formulae, 2012, 18(2): 12-15 |
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| Authors: | LIU Yu-feng WANG Cheng-cheng LIU Yu GAO Fei CHEN Li-jiang |
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| Affiliation: | College of Pharmacy, Liaoning University, Shenyang 110036, China;College of Pharmacy, Liaoning University, Shenyang 110036, China;College of Pharmacy, Liaoning University, Shenyang 110036, China;College of Pharmacy, Liaoning University, Shenyang 110036, China;College of Pharmacy, Liaoning University, Shenyang 110036, China |
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| Abstract: | Objective: To study and optimize smashing tissue extraction technology of total paeony glycoside. Method: Total paeony glycoside was extracted by smashing tissue extractor, the content of paeoniflorin and albiflorin were determined by HPLC,and used it as main indexes.Optimum smashing tissue technology was investigated by L9(34) orthogonal test. Result: Optimum smashing tissue extraction conditions were as follows:Radix paeoniae was dipped in 30 times the amount of water for 6 hours and extracted by smashing tissue extraction for 2 minutes with voltage of 70 V. Conclusion: Smashing tissue extraction method could be used to extract of total paeony glycoside,and it was feasible, simple and rapid and had good application prospect. |
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| Keywords: | total paeony glycoside paeoniflorin albiflorin smashing tissue extraction orthogonal test HPLC |
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