病毒性脑炎脑脊液病毒特异性抗体ELISA检测结果分析

目的探讨儿童病毒性脑炎脑脊液病毒病原学特点及临床特征。方法应用酶联免疫吸附试验（ELISA）疗法埘121例临床诊断为病毒性脑炎患儿的脑脊液进行肠道病毒（EV）、单纯疱疹病毒（HSV）I型和Ⅱ型、EB病毒（EBV）、腺病毒（ADV）及流感病毒（FLuV）特异性IgM抗体检测。结果脑脊液病毒特异性IgM抗体检测阳性75例，阳性率61．98％。肠道病毒阳性35例，居第1位，其次是单纯疱疹病毒I型和Ⅱ型，共17例。肠道病毒感染多见于婴幼儿（6个月～3岁），单纯疱疹病毒感染多见于学龄儿童；肠道病毒感染多见于夏秋季，单纯疱疹病毒感染多见于冬备季，EB病毒、腺痫毒及流感病毒各季节散发。结论肠道病毒是本地区儿童病毒性牺炎感染的主要病原；晒脊液病毒特异性IgM抗体检测町作为早期病原诊断的指标之一。

Results of ELISA detection of IgM in cerebrospinal fluid from children with encephalitis

Abstract] Objective To investigate the pathogenic characteristics of viral encephalitis in cerebrospinal fluid （CSF） from children. Methods IgM antibodies specific to EV, HSV-I, HSV-II, EBV, ADV, and FluV were determined by enzyme linked immunosorbent assay （EI.ISA）. Antibodies were measured in CSF from 121 children with viral encephali tis. Results Of the children tested, 75 （61.98%） had CSF with lgM antibodies specific to viral encephalitis. The most prevalent pathogen was EV （35 patients）, followed by HSV-I （17 patients） and HSV-II （17 patients）. EV infection was commonly seen in infants and young children （6 months to 3 years） while HSV was commonly seen in school-aged chib dren. EV infection usually occurred in the summer and autumn while HSV infection usually occurred in the winter and spring. Conclusion EV is still the leading cause of central nervous system infections in children in this area. Detection ol virus specific IgM antibodies in CSF may facilitate early diagnosis.