| 黄病毒属病毒CODEHOP RT—PCR检测方法的建立 |
| |
| 引用本文： | 胡群,马思杰,孙肖红,李永东.黄病毒属病毒CODEHOP RT—PCR检测方法的建立[J].中国寄生虫病防治杂志,2013(10):878-880. |
| |
| 作者姓名： | 胡群 马思杰 孙肖红 李永东 |
| |
| 作者单位： | [1]大榭出入境检验检疫局,浙江宁波315812 [2]中国检验检疫科学研究院 ,浙江宁波315812 [3]宁波市疾病与预防控制中心,浙江宁波315812 |
| |
| 基金项目： | 国家质检总局科技项目（No.20121K233）. |
| |
| 摘 要： | 目的建立一种能快速检测黄病毒属病毒的CODEHOPRT—PCR方法。方法根据GenBank发丧的不同黄病毒多聚蛋白氯基酸序列，利用CODEHOP方法设计合成一对引物，建立能快速检测黄病毒属所有病毒的CODE～HOPRT—PCR方法，并用3种不同病毒株对该方法进行特异性和灵敏度评价。结果建立的CODEHOPRTPCR能埘黄病毒RNA进行特导性扩增，目的片段的大小（400-500bp）和序列与预期结果相符。该方法对黄病毒恢酸的最小检出量为8Pg。结论建立的CODEHOPRT—PCR方法特异强、灵敏高，可用于黄病毒的榆测。
|
| 关 键 词： | 黄病毒 检测 CODEHOP RT-PCR |
Development of CODEHOP RT-PCR to detect Flavivirus |
| |
| HU Qun,MA Si jieI,SUN Xiao-hong,LI Yong-dong.Development of CODEHOP RT-PCR to detect Flavivirus[J].Chinese Journal of Parasitic Disease Control,2013(10):878-880. |
| |
| Authors: | HU Qun MA Si jieI SUN Xiao-hong LI Yong-dong |
| |
| Institution: | 1. Daa＇ie E.rit Entry Inspection and Quaranti,w Bureau, Da.rie, NingBo, Zhejiang 315812, China; 2. Clinese Academy of Inspection and Quarantine; 3. NingBo Center of Disease Control and Prevention ) |
| |
| Abstract: | Abstract] Objective To develop a rapid method of detecting Flavivirus by RT PCR using consensus degenerate hybrid oligonucleotide primers （CODEHOPs）. Methods In accordance with the amino acid sequences of polyproteins of differ en＊ flaviviruses in GenBank, a pair of primers was designed using CODEHOP, and then CODEHOP RT-PCR was devel oped to rapidly detect all types of flaviviruses. The specificity and sensitivity of this technique were evaluated using 3 dif ferent virus strains. Results CODEHOP RT PCR specifically amplified flavivirus. The size of the target fragment and the sequence coincided with the anticipated result （400-- 500 bp）. The minimum detection of flavivirus RNA was 8 pg. Conclusion C（）DEHOP RT PCR has the advantages of a high specificity and sensitivity and can be used to detect flavivir uses. |
| |
| Keywords: | Flavivirus detection CODEHOP RT PCR |
| 本文献已被 维普 等数据库收录！ |
