醛酮还原酶1-B10在胃癌组织中的表达及其临床意义

目的探讨醛酮还原酶1-BIO在胃癌组织中的表达及其与临床病理特征和预后的关系。方法通过荧光定量PCR法检测36例胃癌组织及其配对癌旁组织中AKRlBl0mRNA的表达量，采用免疫组织化学法检测100例原发胃癌组织及70例非肿瘤胃黏膜组织中AKRlB10蛋白表达情况。结果PCR结果显示，91．7％（33／36）癌旁非肿瘤胃黏膜组织中AKRlB10mRNA的表达高于其配对胃癌组织8．3％（3／36），P=0．000]。100例胃癌组织中33例（33．0％）AKRIBl0蛋白阳性表达，70例非肿瘤胃黏膜上皮中65例（92．9％）AKRlB10的阳性表达。两者比较，差异有统计学意义（Jp=0．000）。胃癌组织中AKRIBIO的表达与患者性别、年龄、肿瘤部位及分化程度无关（p〉0．05），而与肿瘤大小（P=O．000）、浸润深度（P=0．004）、淋巴结转移（P=0．028）、远处转移（P=0．031）和临床TNM分期（P=0．000）有关。AKRlB10阳性表达患者的5年生存率显著高于阴性表达者（60．6％比32．8％，P〈0．01）。结论胃癌组织中AKRlB10的表达下调与胃癌进展有关，提示预后不良。

Expression of aldo-keto reductase family 1 member BIO in gastric cancer tissues and its clinical significance

Objectitv To investigate the association of AKR1B10 expression in gastric cancer tissues with clinicopathologic features and prognosis of gastric cancer patients. Methods Real-time polymerase chain reaction （RT-PCR） was conducted to detect AKR1B10 mRNA expression in gastric cancer and adjacent gastric mucosa tissues （n=36）. AKR1B10 protein expression was measured by immunohistochemistry in primary gastric cancer tissues（n=100） and non-tumorous gastric mucosa tissues （n=70）. Resorts RT-PCR results confirmed that AKR1B10 was significantly down-regulated in gastric cancer tissues compared with that in paired adjacent mucosa8.3%（3/36） vs. 91.7%（33/36）, P=0.000]. Immunohistochemistry revealed that the percentage of AKR1B10 positive specimens in gastric carcinoma was lower than that in normal specimens33.0%（33/100） vs. 92.9%（65/70）, P=0.000]. The frequencies of positive AKR1B10 in patients was significantly correlated with tumor size （P=0.000）, invasive depth （P=0.004）, lymph node metastasis （P=0.028）, distant metastasis （P=0.031 ） and TNM stages （P=0.000）. The 5-year survival rate of positive AKR1B10 group was significantly higher as compared to negative group（60.6% vs. 32.8%, P〈0.01）. Conclusion The down-regulation of AKR1B10 expression in gastric cancer may be associated with the progress of gastric cancer is suggestive of poor prognosis.