| HPLC梯度洗脱法测定防风中4种主要成分含量 |
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| 引用本文： | 李丽,刘元艳,耿立冬,肖永庆. HPLC梯度洗脱法测定防风中4种主要成分含量[J]. 中国中药杂志, 2006, 31(3): 194-196 |
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| 作者姓名： | 李丽 刘元艳 耿立冬 肖永庆 |
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| 作者单位： | 中国中医科学院,中药研究所,北京,100700 |
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| 摘 要： | 目的:在原有防风HPLC含量测定方法基础上改进测定条件。方法:应用HPLC法,以防风中4种主要成分为指标;采用Alltima C18(4.6 mm×150 mm,5μm)色谱柱,以甲醇-水梯度洗脱,流速1.0 mL.min-1。结果:运用梯度洗脱,建立防风中防风色原酮Ⅰ葡萄糖苷,防风色原酮Ⅱ葡萄糖苷,防风色原酮Ⅰ,防风色原酮Ⅲ葡萄糖苷的HPLC含量测定方法,与前方法相比测定结果无显著性差异。结论:HPLC梯度洗脱法测定防风中4种主要成分含量比原方法(两种流动相系统)简便、易于操作。
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| 关 键 词： | 防风 化学成分 含量测定 HPLC 梯度洗脱 |
| 文章编号： | 1001-5302（2006）03-0194-03 |
| 收稿时间： | 2005-07-14 |
| 修稿时间： | 2005-07-14 |
Determination of four components in root of Saposhnikovia divaricata by HPLC gradient elution |
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| LI Li;LIU Yuan-yan;GENG Li-dong;XIAO Yong-qing. Determination of four components in root of Saposhnikovia divaricata by HPLC gradient elution[J]. China Journal of Chinese Materia Medica, 2006, 31(3): 194-196 |
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| Authors: | LI Li LIU Yuan-yan GENG Li-dong XIAO Yong-qing |
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| Affiliation: | Institute of Chinese Materia Media, China Academy of Chinese Medical Sciences, Beijing 100700, China. |
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| Abstract: | Objective: To develop an HPLC method for determination of components in root of Saposhnikovia divaricata.Method: The separation of four components was achieved on a reversed-phase Alltima C18 column with MeOH-H2O gradient elution at a flow rate of 1.0 mL·min-1.Result: Six samples were determined and there is no distinct difference between the contents determined by the new and old methods.Conclusion: The new method is more simple and convenient than the old method. |
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| Keywords: | Saposhnikovia divaricata chemical components assay HPLC gradient elution |
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