A Study of PCR with DNA Extracted from Single Cell Isolated from Histological Sections

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A Study of PCR withDNA Extracted from Single Cell Isolated from Histological Sections

Aim To use and modify the molecular histology technique that was first introduced by Hansmann et al and to investigate the cell origin and the clonality of Hodgkin/Reed-Sternberg (H/R-S) cell. Method Single H/R-S cells were isolated by micromanipulation frcm frozen histological sections of tissues affected by Hoclgkins disease. After DNA was extracted from these cells, PCR was performed with primers designed for β-globin gene that exists in any somatic cells.Results The results showed that a special PCR product (268bp) was found in 12 out of 38 H/R-S cells (lositive rate of 31.6% ) with DNA from the cells micropicked from the frozen sections, whereas no any products was found with the DNA from these cells of formalin-fixed and paraffin-embedded sections. Conclusions The molecular histology technique we used is suitable for detecting the immunoglobulin heavy and light chain gene rearrangement in single H/R-S cells.