CML相关TCRVα亚家族T细胞的克隆性分布特点

 目的 了解慢性粒细胞白血病（CML）患者外周血TCR Vα亚家族T细胞的克隆性分布特点。方法 采用RT-PCR扩增10例CML患者外周血的单个核细胞的TCR Vα29个亚家族基因，分析其TCR Vα亚家族的利用情况。PCR产物进一步经基因扫描分析，了解其CDR3长度以判断T细胞的克隆性。结果 不同病人外周血中所能检测出的Vα亚家族数量不等（1～21个），以Vα3、Vα4、Vα8、Vα10和Vα14为常见，并在Vα1、Vα2、Vα3、Vα4、Vα5、Vα8、Vα10、Vα12、Vα14、Vα15、Vα16、Vα19、Vα21和Vα23中发现克隆性生长T细胞。结论 CML病人外周血T细胞的TCR Vα亚家族选用呈现明显的选择性并出现克隆性T细胞，这可能与机体对白血病相关抗原产生特异性免疫有关，且克隆性增殖Vα亚家族分布以个体特异性为主。

Analysis of Clonal Expansion of TCR Vα Subfamily T Cells Associated with CML

Objective 　To investigate the dist ribution and clonality of TCR Vαsubfamily T cells in patients with chronic myeloid leukemia (CML) . Methods 　The CDR3 size of TCR Vα 29 subfamily genes was amplified in peripheral blood mononuclear cells from 10 cases with CML using RT-PCR ,to observe the usage of TCR Vα repertoire ,the PCR products were further analyzed by genescan technique for detection of the CDR3 size ,to evaluate clonality of TCR Vα T cells. Results 　1～21 Vα subfamily T cells could be identified from different samples in CML cases ,predominantly in Vα3 , 4 , 8 , 10 , 14. Oligoclonal (clonal expansion) T cells were identified in Vα1 , 2 , 3 , 4 , 5 , 8 , 10 , 12 , 14 , 15 , 16 , 19 , 21 and 23 subfamilies. Conclusion 　The selected usage and clonal expansion of TCR Vα subfamily T cells from peripheral blood could be found in patients with CML ,it may be related to CML associated-antigen. Distribution of Vα subfamily clonal expansion displays individual specificity.