Development of a triplex PCR assay for the specific detection of Campylobacter jejuni,Salmonella spp., and Escherichia coli O157:H7 |
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Authors: | Gilbert Carl Winters Debra O'Leary Awilda Slavik Michael |
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Affiliation: | Department of Poultry Science, Center of Excellence for Poultry Science, University of Arkansas, POSC O-310, Fayetteville, AR 72701, USA. |
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Abstract: | A triplex PCR assay was developed and evaluated for efficacy in detecting Campylobacter jejuni, Salmonella spp., and Escherichia coli O157:H7 in a variety of raw and ready-to-eat food products. Following a short enrichment period, artificially contaminated food samples were subjected to a triplex PCR assay, which incorporated published primers for each food pathogen, a protocol for sample collection, and a PCR procedure designed specifically for the assay. The selected primers amplified fragment sizes of 159 bp, 252 bp, and 360 bp for C. jejuni, E. coli O157:H7, and Salmonella spp., respectively. This assay provides specific and reliable results and allows for the cost-effective detection of all three bacterial pathogens in one reaction tube. |
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