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| 隔离肺灌注模型的动物实验研究 | |
| 引用本文： | 辛华,闫继东,高楠,刘威,黄海波.隔离肺灌注模型的动物实验研究[J].中国实验诊断学,2007,11(7):952-953. |
| 作者姓名： | 辛华 闫继东 高楠 刘威 黄海波 |
| 作者单位： | 1. 吉林大学中日联谊医院,吉林,长春,130033 2. 吉林省中医中药研究院 3. 青岛大学医学院附属烟台毓璜顶医院 |
| 摘 要： | 目的 成功建立隔离肺灌注化疗的动物模型。方法 建立兔隔离单肺灌注系统，使用阿霉素进行灌注化疗，并应用荧光分光光度法检测双肺及心、肝、肾组织中阿霉素含量及肺湿／干重量比。结果 隔离灌注肺的阿霉素含量较对照肺及心、肝、肾组织显著升高；而肺湿／干重量比则无明显差别。结论 我们使用的隔离肺灌注系统可在肺组织局部得到较高的化疗药物浓度，且未出现急性肺损伤，使用该模型治疗肺癌及肺转移癌应可取得较好效果。 |
| 关 键 词： | 隔离肺 灌注 阿霉素 肺癌 |
| 文章编号： | 1007-4287（2007）07-0952-02 |
| 修稿时间： | 2006-11-21 |
Exprimental Study of Isolated Lung Perfusion Animal Model |
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| XIN Hua, YAN Ji-dong , GAO Nan,et al..Exprimental Study of Isolated Lung Perfusion Animal Model[J].Chinese Journal of Laboratory Diagnosis,2007,11(7):952-953. | |
| Authors: | XIN Hua YAN Ji-dong GAO Nan |
| Institution: | Deparment of Thoracic Surgery, China-Japan Union Hospital, Jilin University, Changchun Jilin 130031, China |
| Abstract: | Objective To establish the animal model of isolated lung perfusion.Methods Successfully establish a rabbit model of single lung perfusion system.The lung is isolated and the perfusion is limited between the pulmonary artery and pulmonary vein.Use this model and perfuse with Adriamycin(ADM).Then examine the ADM level in the tissue of two lungs,heart,liver and kidney by the fluorescence spectrophotometry.Exmine the wet/dry lung weight.Results The ADM level in the isolated lung is extremly higher than that in the contrast lung,heart,liver,kidney.The wet to dry weight of two lungs have no difference.Conclusion Higher drug level can get in the isolated lung without acute lung injury.This animal model is safe and effective. |
| Keywords: | isolated lung perfusion model animal |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |