Adenovirus‐mediated Interferon‐γ Gene Therapy Induced Human Pancreatic Carcinoma Capan‐2 Cell Apoptosis In Vitro and In Vivo |
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| Authors: | Fa‐Jun Xie Peng Zhao Yi‐Ping Zhang Fei‐Ye Liu Xi‐Lin Nie Ying‐Hui Zhu Xin‐Min Yu Qiu‐Qing Zheng Wei‐Min Mao Hong‐Yang Lu Hong Wei WenLin Huang |
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| Affiliation: | 1. Department of Medical Oncology, Zhejiang Cancer Hospital, , HangZhou, 310022 China;2. Key Laboratory Diagnosis and Treatment Technology on Thoracic Oncology, , HangZhou, 310022 China;3. Department of Medical Oncology, The First Affiliated Hospital, School of Medicine, Zhejiang University, , HangZhou, 310003 China;4. State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat‐sen University, , Gungzhou, 510060 China;5. Department of Oncology, Nanfang Hospital, Southern medical University, , 510515 Guangdong Province, People's Republic of China |
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| Abstract: | Pancreatic cancer is one of the most lethal human malignancies with a very low 5‐year survival rate, which highlights urgent needs for more effective therapeutic strategies. In this study, we examined the potential therapeutic effects of an adenovirus encoding human interferon gamma (Ad‐IFNγ) on pancreatic carcinoma cells Capan‐2 in vitro and in vivo. The results indicated that Ad‐IFNγ could significantly inhibit tumor cell growth via inducing cell apoptosis. After infection, IFNγ expressed durably and stably in xenografts, predominantly in tumor tissue, while much less in blood and liver. Thus, adenovirus‐mediated intratumoral injection of human IFNγ gene could be an effective gene therapeutic system for the treatment of pancreatic carcinoma. Anat Rec, 296:604–610, 2013. © 2013 Wiley Periodicals, Inc. |
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| Keywords: | pancreatic carcinoma adenoviral vector IFNγ gene therapy |
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