The effect of LSD on the surface of neuroepithelial cells

The neuroepithelial cells of 10 control chick embryos and of 22 exposed to lysergic acid diethylamide (LSD) (50 μg/ml) were examined in scanning electron microscopy (SEM). In specimens exposed to LSD, the cells are swollen and their surface loses its cytoplasmic projections. Labelling techniques applied in transmission electron microscopy (TEM) show that ruthenium red attaches to the surface of the neuroepithelial cells in the form of a continuous dark line in both controls and treated specimens. However, when cationized ferritin or lanthanum is used, the label appears in the form of a continuous line in the controls whereas it is discontinuous in specimens exposed to LSD. These observations suggest that LSD alters the components of the neuroepithelial cell surface in the young chick embryo.