| PNH患者红细胞膜缺乏自身限制因子的研究 |
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| 引用本文： | 周凤兰 路金芝. PNH患者红细胞膜缺乏自身限制因子的研究[J]. 中华血液学杂志, 1995, 16(11): 586-588 |
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| 作者姓名： | 周凤兰 路金芝 |
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| 作者单位： | 中国医学科学院基础医学研究所 |
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| 摘 要： | 采用抗Ｃ＿９－Ｓｅｐｈａｒｏｓｅ亲和层析柱，从正常人红细胞膜分离出同种限制因子（ＨＲＦ）。试验表明它有限制未端补体溶血的功能。用细胞亲和层析法从混合的阵发性睡眠性血红蛋白尿症（ＰＮＨ）红细胞中分离出纯而完整的ＰＮＨＩＩＩ型细胞。试验说明纯ＰＮＨＩＩＩ型细胞上缺乏ＨＲＦ。
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| 关 键 词： | 阵发性睡眠性血红蛋白尿，自身限制因子，细胞亲和层析 |
DEFICIENCY OF THE HOMOLOGOUS RESTRICTIONFACTOR IN PAROXYSMAL NOCTURNALHEMOGLOBINURIA |
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| Zhou Fenglan,Lu Jinzhz,Ai Rong. DEFICIENCY OF THE HOMOLOGOUS RESTRICTIONFACTOR IN PAROXYSMAL NOCTURNALHEMOGLOBINURIA[J]. Chinese Journal of Hematology, 1995, 16(11): 586-588 |
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| Authors: | Zhou Fenglan Lu Jinzhz Ai Rong |
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| Abstract: | membrane protein called the homologous restriction factor (HRF) was isolated from human erythrocytes(RBCi with anti-C9 affinity column. It inhibited complementmediated transmembrane channel expression. Because antiHRF treated normal hurnanRBC resembled PNHRBC in susceptibility to reactivelysis, the question arose as to whether PNH-RBC wasdeficient in HRF. Therefore, we separated pure typeIII cells from two PNH patient's whole blood cells.The results showed that in both the cases the affectedRBC lacked HRF protein. |
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| Keywords: | Paroxysmal nocturnal hemoglobinuriaHomologous restriction factorAffinity chromatography |
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