复方配伍共煎对三七中皂苷类成分的影响

目的：探讨复方配伍共煎对三七中皂苷类成分的影响。方法：以三七皂苷R₁、人参皂苷Rg₁,Re,Rb₁以及pH为指标,考察不同配伍复方提取液加热2 h后三七皂苷含量变化及其与pH的相关性。结果：三七与女贞子、三七与土鳖虫、三七与地龙的配伍药液以及缺水蛭脂糖降方药液pH在5.7以上,其三七皂苷保留率均高于90％；三七与丹参、三七与赤芍、三七与桔梗、三七与牛蒡子的配伍药液pH在4.5～5.5,其三七皂苷保留率在60％～85％；三七与水蛭的配伍药液,pH下降至3.4,三七皂苷保留率仅为38.4％；采用0.1％NaOH溶液调节脂糖降方药液的pH至6.3,三七皂苷保留率显著提高至97％。结论：与三七配伍的其他中药提取液的pH值大小,对三七皂苷的稳定性有重大影响。

Effect compound decoction on notoginsenosides in Panax notoginseng

OBJECTIVE: To explore the effect of compound decoction on notoginsenosides in Panax notoginseng. METHOD: Notoginsenoside R1, Rg1, Re, Rb1 and pH were used as the parameters to investigate the changes on the content of notoginsenosides in different compound extractions by heating for two hours and their correlation with pH. RESULT: When the pH values of solution of P. notoginseng with Fructus ligustri, P. notoginseng with Eupolyphaga seu steleophaga, P. notoginseng with Pheretima asiatica, and Zhitangjiang Fang (free of Hirudo) were rept higher than 5.7, the reserved rate (RR) of notoginsenside were higher than 90%; When the pH values of decoetion of P. notoginseng with Salvia miltiorrhiza, P. notoginseng with Paeonia lactiflora, P. notoginseng with Platycodon grandiflorum, P. notoginseng with Arctium lappa were kept 4.5-5.5, their RR of notoginsenside were 60% - 85%; When the pH values of the decotction of P. notoginseng with Hirudo nipponica was decreased to 3.4, its RR of of notoginsenside was 38.4%; When the pH values of Zhitangjiang Fang extraction was regulated by 0.1% NaOH solution to pH 6. 3, and the RR of notoginsenside increased to 97%. CONCLUSION: The pH of other Chinese herbal medicines extraction with P. notoginseng compound is a critical effect on the stability and yields of notoginsensides.