低频电磁场干预组织工程化骨修复兔股骨骨缺损的实验研究

Experimental study on using tissue-engineered bone exposed with low-frequency electromagnetic fields to repair femur bone defect in rabbits

Objective To explore the effects of low - frequency electromagnetic fields on the osteogenetic ability of tissue-engineered bone and the effect of repairing segmental bone defect of the middle segment of femur in rabbits. Methods Bone mesenchymal stem cells (BMSCs) were seeded on beta tricalcium phosphate (β-TCP) scaffold to construct an engineered bone graft which was exposed with or without low-frequency electromagnetic fields. CCK-8, ALP, and qPCR tests were applied to assess proliferation and differentiation. Rabbit femur segmental bone defect model was established to evaluate the efficacy of the electromagnetic fields exposed engineered construct for osteogenesis. Results The proliferation rate of BMSCs in the engineered bone grafts exposed to electromagnetic fields was increased. Electromagnetic field exposition promoted the gene expression and ALP activity of BMSCs. In vivo study demonstrated increased bone formation was seen in the electromagnetic fields exposed groups. Conclusion Electromagnetic fields exposure can enhance the osteogenic potentials of the engineered BMSCs/β-TCP constructs. The BMSCs/β-TCP constructs exposed to electromagnetic fields can promote in vivo bone regeneration.