Foxp3^＋、CD4^＋、CD25^＋调节性T淋巴细胞与慢性阻塞性肺疾病相关性的研究

目的：探讨慢性阻塞性肺疾病（COPD）调节性T淋巴细胞（Treg）Foxp3^＋、CD4^＋和CD25^＋的变化及3种细胞因子白介素-10（IL-10）、肿瘤坏死因子-α（TNF-α）、转化生长因子-β（TGF-β）与CD4^＋Treg的相关性。方法：采用流式细胞术检测外周血CD4^＋Treg数量,采用酶联免疫法（ELISA）测定血清IL-10、TNF-α、TGF-β水平。结果：COPD急性加重期、缓解期外周血Foxp3^＋、CD4^＋、CD25^＋T淋巴细胞（CD4^＋Treg）占单核细胞百分比明显增高,与正常对照组相比差异有统计学意义（P〈0.05）,缓解期略高于急性加重期,两者相比无显著性差异;COPD急性加重期组患者血清IL-10水平低于正常对照组,缓解期组低于急性加重期组,3组比较有显著性差异（P〈0.01）。COPD急性加重期及缓解期组TNF-α水平明显升高,均高于正常对照组（P〈0.01）。COPD急性加重期血清TGF-β浓度高于缓解期及正常对照组,有显著性差异,但缓解期与正常对照组相比较无显著性差异。正常对照组IL-10与CD4^＋Treg成正相关（r值为0.655,P〈0.05）,COPD急性加重期TNF-α与CD4^＋Treg成正相关（r值为0.4,P〈0.05）。结论：CD4^＋Treg可能参与COPD的发病及急性加重过程。

Dependablity Study about Foxp3+ CD4+ CD25+ T Regulatory Lymphocyte to Chronic Obstructive Pulmonary Disease

Objective：To investigate the change of Foxp3^＋ ,CD4^＋ and CD25^＋ T regulatory lymphocyte （CD4^＋ T reg）about chronic obstructive pulmonary disease, and the relationship IL- 10. TNF-α, TGF-β and CD4^＋ T reg. Methods： The number of CD4^＋ T reg was detected among by use of flow cytometry. The content of serum IL -10,TNF-α and TGF-β were determined by enzymelinked immunosorbent assay. Results： The percentage of CD4^＋ Treg on peripheral blood monocyte in COPD acute exacerbation and eatabasis was significantly higher than normal control group （P〈0.05）, no significant difference between COPD acute exacerbation and catabasis. The level of IL-10 in serum of COPD acute exacerbation phase and eatabasis group lower than normal control group, significant difference was found compared the three group （P〈0.05）. The level of TNF-α in serum of COPD acute exacerbation phase and catabasis significantly higher than that in normal control group（ P〈0. 01 ）. The content of TGF-β in serum of COPD acute exacerbation was higher than that in normal control group and COPD catabasis group, but compared COPD eatabasis and normal control group, no significant difference was found. Positively correlate between IL- 10 and CD4^＋ Treg in normal control group （r=0.665,P〈0.05）. positively correlate hetween TNF-α and CD4^＋ Treg in COPD acute exacerbation group （r=0.4,P〈0. 05）. Conclusion： CD4^＋ Treg maybe participate the procedure of COPD morbidity and acute exacerbation.