红景天对睡眠剥夺大鼠学习记忆的影响及其机制探索

目的:探讨红景天对睡眠剥夺大鼠学习记忆功能的影响及其可能机制。方法:用小平台水环境法建立睡眠剥夺模型,每组半数行Y型电迷宫测试,并检测海马和额叶组织中超氧化物歧化酶(SOD)活力及丙二醛(MDA)含量,半数利用免疫组化法检测海马区胆碱乙酰转移酶(ChAT)及胶质纤维酸性蛋白(GFAP)表达。结果:Y型电迷宫测试显示,与模型组相比,红景天组全天总反应时间(TRT)和错误反应次数(EN)均较低(P<0.05)。与模型组相比,红景天组海马组织和额叶组织SOD活力均较高(P<0.05)。与模型组相比,红景天组海马组织MDA含量较低(P<0.05),额叶组织MDA含量与模型组差别无统计学意义(P>0.05)。ChAT表达检测表明,与模型组相比,红景天组阳性细胞着色较深,轮廓较清晰,排列较整齐,各亚区阳性细胞数量较多(P<0.05)。GFAP表达检测显示,与模型组相比,红景天组阳性细胞着色较浅,细胞体积较小,突起较短较细,侧枝较少,阳性细胞数量较少(P<0.05)。结论:红景天对睡眠剥夺后大鼠脑组织氧化损伤及神经元损伤有保护作用,可缓解睡眠剥夺造成的学习记忆功能的下降。

Effects of Rhodiola Rosea on learning and memory in rats after sleep deprivation and the dissection of its mechanism

AIM. To investigate the effects and possible mechanism of Rhodiola Rosea （RR） on learning and memory in rats after sleep deprivation. METHODS. Sleep deprivation was induced by the flower pot technique. Half of eachgroup went through Y-maze test and were detected for superoxide dismutase （SOD） activity and malondialdehyde （MDA） content in hippocampus and frontal lobe. The other half were detected for expression of choline acetyl transferase（CHAT） and glial fibrillary acidic protein （GFAP） in hippocampus by immunohistochem- istry technique. RESULTS： In Y-maze test, total reaction time （TRT） was shorter and error numbers （EN） were less （P~0.05） in RR group than in model group. SOD activity in both hippocampus and frontal lobe was higher （P〈0.05） and MDA content in hippocampus was lower （P〈0.05） in RR group than in model group. In immunohistochemistry detection, ChAT expression positive cells were in larger quantity and colored at darker profile with clea- rer outline and more regular arrangement （P〈 0.05） in RR group than in model group. GFAP expression positive cells were in smaller quantity and colored at lighter mode with smaller volume and fewer and shorter neurite and branches （P〈 0.05） in RR group than those in model group. CONCLUSION. RR can protect brain from oxidative damage and neuron injury as well as alleviate the impairment on learning and memory capability caused by sleep deprivation.