聚乳酸乙醇酸共聚物与纳米羟基磷灰石共混制备组织工程纤维环支架的实验研究

目的探讨以聚乳酸乙醇酸共聚物(poly(lactic-co-glycolic acid),PLGA)与纳米羟基磷灰石(hydroxyapa-tite,HA)为原料,利用静电纺丝的技术制备出的三维多孔支架构建组织工程纤维环的可行性。方法将PLGA与HA溶解于丙酮中,配制20%(w/v)的溶液,磁力搅拌均匀后,进行静电纺丝,制备三维多孔支架。支架通过扫描电镜和磷酸盐缓冲液降解(phosphate buffered saline,PBS)的方式,观察支架的形貌和降解性能;利用CCK-8和伊红染色的方法观察支架的生物相容性。结果支架具有良好的纤维形貌,直径均匀,扫描电镜下可见HA结晶。支架在磷酸盐缓冲液中降解速度较快,8周时支架失重可达40%,表现出良好的降解性能。细胞毒性试验和伊红染色均表明细胞在支架上的生长情况较好,支架具有良好的生物相容性。结论 PLGA与HA共混制备的纳米纤维支架具有良好的空间结构和生物相容性,因此该支架是一种良好的组织工程纤维环支架材料。

Experiment study on tissue-engineered annulus fibrosus scaffold prepared with PLGA and HA

Objective To investigate the feasibility of tissue-engineered annulus fibrosus scaffold prepared with PLGA and HA through the electrostatic spinning method. Methods PLGA and HA were dissolved in the acetone to form 20% （w/v） solution and stired to homogeneous. The scaffold was prepared through the electrostatic spinning meth- od. The morphology and degradation of scaffold were observed by scanning electron microscopy and degradation in phos- phate buffered saline. The biocompatibility of the scaffold was observed by CCK-8 and eosin staining. Results The mor- phology of scaffold were fibers with uniform diameter, and the crystal HA on the nanofibers could be observed by scan- ning electron microscopy, The fibers degradated quickly in phosphate buffered saline. At 8 week, the mass loss could be up to 40%. This result showed that the degradation of the scaffold is well. The CCK-8 and eosin staining proved that the annulus fibrosus cells grew well on the scaffold. This suggested that the scaffold is biocompatibility. Conslusion The nanofiber scaffold is advantaged on its well spatial structure and excellent bioeompatiblity. The nanofiber scaffold is a promising tissue-engineered annulus fibrosus scaffold.