Ouabain诱导沙鼠蜗外电诱发听性脑干反应变化研究

目的沙鼠耳蜗圆窗应用毒毛旋花甙G(Ouabain),观察沙鼠蜗外电诱发听性脑干反应(EABR)的变化。方法显微镜下将浸有50μl Ouabain(1 mM)的明胶海绵置于沙鼠耳蜗圆窗膜表面,观察应用Ouabain 24 h、96 h后听觉脑干反应(ABR)、耳蜗微音电位(CM)、蜗外电诱发听性脑干反应(EABR)阈值变化。结果沙鼠圆窗应用Ouabain 24h、96 h后ABR、EABR阈值明显升高,差异具有统计学意义(P<0.05),CM阈值无明显变化,差异无统计学意义(P>0.05)。结论沙鼠圆窗膜表面应用50μl Ouabain(1 mM)24 h、96 h后可导致沙鼠ABR、EABR阈值明显升高,而CM阈值无明显变化。

Ouabain. The study for the Gerbil electrically auditory brainstem responses change induced by

Objective To observe the Gerbil electrically audiotory brainstem responses change after Ouabain was applied to the round window of the gerbil. Methods A piece of gelfoam immerged with Ouabain solution （50 μl mM） was used to cover on the round window membrane of the cochlea. After applying ouabain in the cochlea for 24 h and 96 h, the threshold changes of audiotory brainstem responses （ ABR）, Cochlear microphanics （ CM）, electrically audiotory brainstem responses （EABR） were observed. Results Ouabain application for 24 h and 96 h induced the threshold of ABR and EABR to increase obviously. The difference had statistical significance（ P 〈0.05）. But the threshold of CM remained no obvious change, the difference had no statistical difference （ P 〉 0.05 ）. Conclusion Ouabain application on the round window membrane of the gerbil cochlea for 24 h and 96h may make the threshold of ABR and EABR increase, but the threshold for CM had no obvious change.