食管癌血清和尿液中血管内皮生长因子水平的变化及其意义

目的检测食管癌患者术前血清和尿液中血管内皮生长因子(VEGF)水平,探讨VEGV与食管癌的关系及其临床意义.方法应用酶联免疫吸附法(ELISA)对82例食管癌患者术前血清和尿液中VEGF水平进行测定,并对其进行对照分析.结果食管癌患者术前血清和尿液VEGF水平分别为(92.81±5.86)ng/L和(277.92±22.71)ng/L,明显高于正常人组(P＜0.01).食管癌组术前尿液VEGF水平与肿瘤的分化程度、侵袭程度、临床分期及淋巴结转移等密切相关(P＜0.05);且术前尿液组的VEGF水平明显高于术前血清组(P＜0.01).结论VEGF可能参与食管癌的发生、发展和转移过程的调控,尿液VEGF含量的增强,预示食管癌的转移和预后不良.

Change in vascular endothelial growth factor level in serum and urine of esophageal carcinoma patients

OBJECTIVE: The purpose of this study was to determine the serum and urine vascular endothelial growth factor (VEGF) level in patients with esophageal carcinoma and to elucidate their significance of pre-operative VEGF levels. METHODS: Urine VEGF level was measured by sandwich ELISA in 82 esophageal carcinoma patients and 15 healthy individuals. RESULTS: The serum and urine VEGF levels (mean +/- s, ng/L) in esophageal carcinoma patients were 92.81 +/- 5.86 and 277.92 +/- 22.71 which were significantly higher than those of healthy subjects (16.01 +/- 3.46 and 15.27 +/- 4.22, P < 0.01). The pre-operatiive urine VEGF level in esophageal cancer patients were significantly higher in advanced stage of the disease (P < 0.01). As to the depth of invasion (T-factor), the urine VEGF level of T4 invasive lesions were significantly higher than those with T1-T3 invasion (P < 0.01). The same was also true in esophageal carcinoma having lymph node metastasis than that without (P < 0.05). The urine VEGF level was significantly higher than the serum VEGF level (P < 0.01). CONCLUSION: VEGF plays an important role in the growth and metastasis of esophageal cancer.