高压氧治疗对大鼠损伤后的脑组织中巢蛋白表达的影响

目的 探讨高压氧治疗对损伤后脑组织保护作用的可能机制.方法 将90只雄性SD大鼠按随机数字表法随机分成假手术组、外伤组、高压氧治疗组,各组再分为伤后1 d、3 d、7 d、14 d和21 d五亚组(n=6);按改良的Feeney法制作重型脑损伤模型;免疫组化法测定所有大鼠受伤脑组织中巢蛋白的表达.结果 外伤后大鼠伤侧脑皮层中巢蛋白表达水平随伤后时间延长而逐渐升高,在伤后14 d达高峰,以后逐渐下降.受伤脑组织中巢蛋白的表达水平高压氧治疗各亚组均明显高于外伤各相应亚组(P<0.01),而外伤各亚组又明显高于假手术组(P<0.01).结论 高压氧治疗上调脑组织中巢蛋白表达可能与其对受伤后脑组织有保护作用有关.

Effect of hyperbaric oxygen therapy on nestin expression in injured cerebral tissues of rats with severe brain trauma

Objective To investigate effect of hyperbaric oxygen therapy on nestin expression in the injured cerebral tissue of the rats with severe brain trauma in order to explore its neuroprotective mechanism. Methods Ninety SD male rats were randomly divided into sham operation group, trauma group and hyperbaric oxygen therapy group, of which, each group was redivided into 5 subgroups of 6 animals each according to 1, 3, 7, 14 and 21 days after the trauma. The model of cerebral injury was made by improved Feeney method in both the trauma and hyperbaric oxygen therapy groups. The nestin expression in the injured cerebral tissues was detected by immunohistochemical technique. Results The levels of nestin expression were gradually increased with the lapse of time after the trauma, reached the top 14 days after the trauma and then decreases in both the trauma and therapy groups, between which there were significant differences in the levels of nestin expression 1, 3, 7, 14 and 21 days after the trauma ( P <0.01). The levels of nestin expression in the therapy group was significantly higher than that in the trauma group ( P <0.01), which was significantly higher than that in the sham operation group ( P <0.01). Conclusions It is suggested that the neuroprotective effect of hyperbaric oxygen on the injured cerebral tissues may be produced by the up-regulation of nestin expression in the injured cerebral tissues because nestin may promote the proliferation and differentiation of the endogenic neural stem cells.